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GIF Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Analysis in human stomach and skin tissues. Corresponding GIF RNA-seq data are presented for the same tissues.
Western Blot: GIF Antibody [NBP1-81610] - Analysis in control (vector only transfected HEK293T lysate) and GIF over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human stomach shows strong cytoplasmic positivity in Parietal cells.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GIF Antibody [NBP1-81610] - Staining of human kidney shows no positivity in cells in tubules as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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GIF Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPSNP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CBLIF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GIF Protein (NBP1-81610PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-81610 in the following applications:

Publications
Read Publication using NBP1-81610.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GIF Antibody

  • gastric intrinsic factor (vitamin B synthesis)
  • GIF
  • IF
  • IFIntrinsic factor
  • IFMH
  • IFMHgastric intrinsic factor
  • INF
  • TCN3

Background

GIF is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GIF Antibody (NBP1-81610)(1)

We have publications tested in 2 applications: Immunohistochemistry, Immunohistochemistry-Frozen.


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Immunohistochemistry
(1)
Immunohistochemistry-Frozen
(1)
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Review for GIF Antibody (NBP1-81610) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse and mouse stomach.

Reviews using NBP1-81610:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse and mouse stomach
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GIF NBP1-81610
Enlarge
5
reviewed by:
Feng Li
ICC Mouse and mouse stomach 05/16/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedStomach tissue
SpeciesMouse and mouse stomach
LotR38115

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Recent Reviews

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5
1
4
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1
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Feng Li
05/16/2017
Application: ICC
Species: Mouse and mouse stomach

Bioinformatics

Gene Symbol CBLIF