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GEN1 Antibody

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Western Blot: GEN1 Antibody [NBP2-30783] - Analysis in human cell line RH-30.
Western Blot: GEN1 Antibody [NBP2-30783] - Analysis in human cell line RH-30.
Immunocytochemistry/ Immunofluorescence: GEN1 Antibody [NBP2-30783] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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GEN1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SILSVKESCIANSGSDCTSHLSKDLPGIPLQNESRDSKILKGDQLLQEDYKVNTSVPYSVSNTVVKTCNVRPPNTALDHSRKVDMQTTRKILMKKSVCLDRHSSDEQSAPVFGKAKYTTQRMKHSSQKHNSSHFKESGHNKLSSPKI
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GEN1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GEN1 Protein (NBP2-30783PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GEN1 Antibody

  • DKFZp781F0986
  • EC 3.1
  • flap endonuclease GEN homolog 1
  • FLJ40869
  • Gen homolog 1, endonuclease (Drosophila)
  • Gen

Background

Endonuclease which cleaves flap structures at the junction between single-stranded DNA and double-strandedDNA. Specific for 5'-overhanging flap structures in which the 5'-upstream of the flap is completely double-stranded.Prefers the blocked-flap structures similar to those occurring at replication forks, in which the 5' single-strandoverhang of the flap is double-stranded

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GEN1
Uniprot