![Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Western-Blot-NBP2-45810-img0013.jpg "Western Blot: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis (35 ug) from 3 different cell lines by using anti-GATA3 monoclonal antibody at 1:200.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | OTI8H4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 155-443 of human GATA3 (NP_002042) produced in E.coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GATA3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 47.9 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA, 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-45810 | Applications | Species |
|---|---|---|
| Przepiorski A, Sander V, Tran T et al. A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells Stem Cell Reports 2018-08-14 [PMID: 30033089] (IHC-P, Human) | IHC-P | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 12/08/2021 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for GATA-3 Antibody (NBP2-45810)Find related products by research area.
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Read full blog post. |
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Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an... Read full blog post. |
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| Gene Symbol | GATA3 |
![Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Mouse lung tissue section stained with GATA-3 antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/GATA-3-Antibody-OTI8H4-Immunohistochemistry-Paraffin-NBP2-45810-img0014.jpg "Immunohistochemistry-Paraffin: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Mouse lung tissue section stained with GATA-3 antibody. IHC-P image submitted by a verified customer review.")
![Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Flow cytometric Analysis of penetrated MCF-7 cells, compared to a nonspecific negative control antibody, (Blue). The right is HUVEC cells as negtive control (1:100).](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Flow-Cytometry-NBP2-45810-img0011.jpg "Flow Cytometry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Flow cytometric Analysis of penetrated MCF-7 cells, compared to a nonspecific negative control antibody, (Blue). The right is HUVEC cells as negtive control (1:100).")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0001.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0002.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human breast tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human colon tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0003.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human colon tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human Kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0004.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human Kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0005.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human kidney tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human endometrium tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0006.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Adenocarcinoma of Human endometrium tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0007.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0008.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Carcinoma of Human prostate tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human bladder tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0009.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human bladder tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human lymphoma tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.](http://images.novusbio.com/fullsize/GATA-3-Antibody-8H4-Immunohistochemistry-NBP2-45810-img0010.jpg "Immunohistochemistry: GATA-3 Antibody (OTI8H4) [NBP2-45810] - Analysis of Human lymphoma tissue. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120C for 3 minutes.")
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![Western Blot T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images2/T-bet_MAB5385_Western_Blot_6233.jpg)
![Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images2/T-bet_MAB5385_Flow_Cytometry_8529.jpg)
![Intracellular Staining by Flow Cytometry T-bet/TBX21 Antibody (525803) [Unconjugated]](https://images.novusbio.com/images/antibody/mab5385_human-t-bet-mab-clone-525803-intracellular-staining-by-flow-cytometry-2932024154136.jpg)
![Western Blot GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Western_Blot_5199.jpg)
![Immunocytochemistry GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-3_AF2605_Immunocytochemistry_6486.jpg)
![Simple Western GATA-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA3_AF2605_Simple_Western_16988.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/IL-5_M5000_ELISA_237.jpg)
![N/A IL-5 [HRP]](https://images.novusbio.com/images2/DATA_IL5_M5000_ELISA_935.jpg)
![N/A IL-13 [Biotin]](https://images.novusbio.com/images2/DATA_IL13_DY413_ELISA_2010.jpg)
![Bioactivity IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFNgamma_285IF_2848.jpg)
![SDS-PAGE IFN-gamma [Unconjugated]](https://images.novusbio.com/images2/IFN-gamma_285-IF_230.jpg)
![SEC-MALS IFN-gamma [Unconjugated]](https://images.novusbio.com/images/285-if_recombinant-human-ifn-gamma-protein-sec-mals-1520239228..jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Western Blot GATA-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA-2_AF2046_Western_Blot_5163.jpg)
![Simple Western GATA-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA2_AF2046_Simple_Western_16938.jpg)
![Western Blot GATA-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/GATA2_AF2046_Western_Blot_22630.jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_729.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/DATA_Kallikrein_3_DKK300_ELISA_730.jpg)
![N/A Kallikrein 3/PSA [HRP]](https://images.novusbio.com/images2/Kallikrein_3_DKK300_ELISA_124.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
