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Fumarylacetoacetate hydrolase Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] - Staining in human liver and pancreas tissues using anti-FAH antibody. Corresponding FAH RNA-seq data are ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] - Staining of human cerebral cortex, liver, pancreas and testis using Anti-FAH antibody NBP2-48736 (A) ...read more
Independent Antibodies: Western Blot: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] - Analysis using Anti-FAH antibody NBP2-48736 (A) shows similar pattern to independent antibody NBP2-48807 (B).
Immunocytochemistry/ Immunofluorescence: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] - Staining of human cell line Hep G2 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] - Staining of human liver using Anti-FAH antibody NBP2-48736.
Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] -Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Fumarylacetoacetate hydrolase Antibody [NBP2-48736] -Staining of human skin shows no positivity in squamous epithelial cells as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Fumarylacetoacetate hydrolase Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: INLSVNLKGEGMSQAATICKSNFKYMYWTMLQQLTHHSVNGCNLRPGDLLASGTISGPEPENFGSMLELSWKGTKPIDLGNGQTRKFLLDGD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FAH
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Fumarylacetoacetate hydrolase Recombinant Protein Antigen (NBP2-48736PEP)

Reactivity Notes

Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fumarylacetoacetate hydrolase Antibody

  • beta-diketonase
  • EC 3.7.1.2
  • FAA
  • fumarylacetoacetase
  • fumarylacetoacetate hydrolase (fumarylacetoacetase)
  • Fumarylacetoacetate hydrolase

Background

The Fumarylacetoacetate hydrolase gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FAH