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FoxP3 Antibody (376209R) [Unconjugated]

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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Monoclonal Antibody (Catalog # MAB8970) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Mouse/Rat FoxP3 Monoclonal Antibody (Catalog # MAB8970) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, CyTOF-ready
Clone
376209R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8970U-100UG
Alexa Fluor 405
IC8970V-100UG
Alexa Fluor 488
IC8970G-100UG
Alexa Fluor 532
FAB8970X
Alexa Fluor 594
IC8970T-100UG
Alexa Fluor 700
IC8970N-100UG
Alexa Fluor 750
IC8970S-100UG
Allophycocyanin
FAB8970A
Allophycocyanin/Cy7
FAB8970APCCY7
Biotin
FAB8970B
CoraFluor 1
FAB8970CL1
DyLight 350
FAB8970D
DyLight 405
FAB8970E
DyLight 488
FAB8970K
DyLight 550
FAB8970L
DyLight 594
FAB8970M
DyLight 650
FAB8970W
DyLight 680
FAB8970Y
DyLight 755
FAB8970Z
FITC
FAB8970F
HRP
FAB8970H
Janelia Fluor 525
FAB8970JF525
Janelia Fluor 549
FAB8970I
Janelia Fluor 646
FAB8970J
Janelia Fluor 669
FAB8970JF669
PE/Atto594
FAB8970PEATT594
PE/Cy5.5
FAB8970PECY55
PE/Cy7
FAB8970PECY7
PerCP
FAB8970C
mFluor Violet 450 SE
FAB8970MFV450
mFluor Violet 500 SE
FAB8970MFV500
mFluor Violet 610 SE
FAB8970MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8970-SP
Carrier Free
IC8970N-100UG
Carrier Free
IC8970G-100UG
Carrier Free
IC8970V-100UG
Carrier Free
IC8970U-100UG
Carrier Free
IC8970T-100UG
Carrier Free
IC8970S-100UG
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FoxP3 Antibody (376209R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E.coli-derived recombinant human FoxP3
Gln105-Lys200
Accession # Q9BZS1
Specificity
Detects human FoxP3 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publication using
MAB8970 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxP3 Antibody (376209R) [Unconjugated]

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (MAB8970)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody (MAB8970). (Showing 1 - 2 of 2 FAQ).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Secondary Antibodies

 

Isotype Controls

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Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.
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