![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0007.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.")
| Reactivity | Hu, Mu, Rt, Po, Bv, SqSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC |
| Clone | 83N7F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | FOXO1A |
| Localization | Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus. |
| Predicted Species | Porcine (90%), Bovine (91%), Squirrel (91%). Backed by our 100% Guarantee. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXO1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 08/23/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for FoxO1/FKHR Antibody (NBP2-31376)Find related products by research area.
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FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunocytochemistry-Immunofluorescence-NBP2-31376-img0009.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0011.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0016.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Western-Blot-NBP2-31376-img0004.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Immunohistochemistry-Paraffin-NBP2-31376-img0010.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0013.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0014.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Flow-Cytometry-NBP2-31376-img0015.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Simple-Western-NBP2-31376-img0012.jpg "Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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