![Western Blot: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Western-Blot-NBP2-80741-img0002.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 83N7F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | FOXO1A |
| Localization | Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXO1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for FoxO1/FKHR Antibody (NBP2-80741)Find related products by research area.
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FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXO1 |
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80741-img0003.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x. Image from the standard format of this antibody.")
![Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80741-img0005.jpg "Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80741-img0009.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Western Blot: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Western-Blot-NBP2-80741-img0001.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.")
![Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla. Image from the standard format o](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80741-img0004.jpg "Immunohistochemistry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla. Image from the standard format o")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80741-img0006.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80741-img0007.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80741-img0008.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.")
![Simple Western: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-83N7F8-Azide-and-BSA-Free-Simple-Western-NBP2-80741-img0010.jpg "Simple Western: FoxO1/FKHR Antibody (83N7F8) - Azide and BSA Free [NBP2-80741] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.")
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