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FOXI1 Antibody

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Western Blot: FOXI1 Antibody [NBP2-16518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FOXI1 Antibody [NBP2-16518] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at ...read more
Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody [NBP2-16518] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: FOXI1 Antibody [NBP2-16518] - Mouse colon. FOXI1 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: FOXI1 Antibody [NBP2-16518] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Western Blot: FOXI1 Antibody [NBP2-16518] - arious whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Western Blot: FOXI1 Antibody [NBP2-16518] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FOXI1 antibody [N2C3] diluted at 1:1000. The HRP-conjugated ...read more
Immunohistochemistry-Paraffin: FOXI1 Antibody [NBP2-16518] - Huh7 xenograft, using FOXI1 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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FOXI1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human FOXI1. The exact sequence is proprietary.
Predicted Species
Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXI1
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: ZebraFish (89%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for FOXI1 Antibody

  • FKHL10HFH3
  • forkhead box I1
  • forkhead box protein I1
  • forkhead-like 10
  • forkhead-related activator 6
  • Forkhead-related protein FKHL10
  • Forkhead-related transcription factor 6
  • FREAC-6
  • FREAC6FKH10
  • Hepatocyte nuclear factor 3 forkhead homolog 3
  • HFH-3
  • HNF-3/fork-head homolog 3
  • HNF-3/fork-head homolog-3
  • MGC34197

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FOXI1
Uniprot