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FoxF1 Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by ...read more
Loss of FOXF1 promotes fundamental cellular processes in LR-MSCs. (A) mRNA was isolated from fibrotic and non-fibrotic LR-MSCs derived from bronchoalveolar lavage fluid of transplant patients, and FOXF1 expression was ...read more
Loss of FOXF1 promotes fundamental cellular processes in LR-MSCs. (A) mRNA was isolated from fibrotic and non-fibrotic LR-MSCs derived from bronchoalveolar lavage fluid of transplant patients, and FOXF1 expression was ...read more
ATX-dependent cell migration in FOXF1-silenced LR-MSCs. (A) LR-MSCs were transfected with scrambled or FOXF1-specific siRNA. 24 h later, these LR-MSCs were transfected with scrambled or ATX-specific siRNA. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Catalog# & Formulation Size Price
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FoxF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FoxF1
Met154-Met379
Accession # Q12946
Specificity
Detects human  FoxF1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXF1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxF1 Antibody [Unconjugated]

  • FKHL5
  • FKHL5MGC105125
  • forkhead box F1
  • forkhead box protein F1
  • Forkhead, drosophila, homolog-like 5
  • Forkhead-related activator 1
  • Forkhead-related protein FKHL5
  • Forkhead-related transcription factor 1
  • FoxF1
  • FREAC-1
  • FREAC1ACDMPV

Background

FoxF1 belongs to a large family of proteins that share a common forkhead/winged helix DNA binding domain. FoxF1 is implicated in pulmonary morphogenesis, specifically in development of the lung mesenchyme. Experiments in mice indicate that haploinsufficiency of FoxF1 can lead to perinatal lethality due to pulmonary abnormalities. In addition expression of FoxF1 can be induced by hedgehog ligands and appears to regulate expression of BMP-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxF1 Antibody (AF4798)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 7 applications: ChIP, ICC, IHC, IHC-P, Immunohistochemistry, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(1)
ICC
(1)
IHC
(3)
IHC-P
(3)
Immunohistochemistry
(2)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(6)
Xenopus
(2)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using AF4798 Applications Species
Gomez-Arroyo, J;Houweling, AC;Bogaard, HJ;Aman, J;Kitzmiller, JA;Porollo, A;Dooijes, D;Meijboom, LJ;Hale, P;Pauciulo, MW;Hong, J;Zhu, N;Welch, C;Shen, Y;Zacharias, WJ;McCormack, FX;Aldred, MA;Weirauch, MT;Graf, S;Rhodes, C;Chung, WK;Whitsett, JA;Martin, LJ;Kalinichenko, VV;Nichols, WC; Role of Forkhead box F1 in the Pathobiology of Pulmonary Arterial Hypertension bioRxiv : the preprint server for biology 2024-09-22 [PMID: 39345371] (Immunohistochemistry, Human) Immunohistochemistry Human
Shirazi, SP;Negretti, NM;Jetter, CS;Sharkey, AL;Garg, S;Kapp, ME;Wilkins, D;Fortier, G;Mallapragada, S;Banovich, NE;Wright, CVE;Frank, DB;Kropski, JA;Sucre, JMS; Bronchopulmonary Dysplasia with Pulmonary Hypertension Associates with Loss of Semaphorin Signaling and Functional Decrease in FOXF1 Expression bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39253423] (Immunohistochemistry, Human) Immunohistochemistry Human
Edwards, NA;Kashyap, A;Warren, A;Agricola, Z;Kenny, AP;Shen, Y;Chung, WK;Zorn, AM; Disrupted endosomal trafficking of the Vangl-Celsr polarity complex causes congenital anomalies in trachea-esophageal separation bioRxiv : the preprint server for biology 2023-10-11 [PMID: 37873300] (IHC, Xenopus) IHC Xenopus
Olena A. Kolesnichenko, Hannah M. Flood, Yufang Zhang, Vladimir Ustiyan, Hayde K. Cuervo Jimenez, Tanya V. Kalin et al. Endothelial progenitor cells derived from embryonic stem cells prevent alveolar simplification in a murine model of bronchopulmonary dysplasia Frontiers in Cell and Developmental Biology 2023-06-09 [PMID: 37363726]
Bian, F;Lan, YW;Zhao, S;Deng, Z;Shukla, S;Acharya, A;Donovan, J;Le, T;Milewski, D;Bacchetta, M;Hozain, AE;Tipograf, Y;Chen, YW;Xu, Y;Shi, D;Kalinichenko, VV;Kalin, TV; Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling Nature communications 2023-05-04 [PMID: 37137915] (Western Blot, Human) Western Blot Human
Susa K, Kobayashi K, Galichon P et al. ATP/ADP biosensor organoids for drug nephrotoxicity assessment Frontiers in cell and developmental biology 2023-03-02 [PMID: 36936695]
Liu X, Geng Y, Liang J et al. HER2 drives lung fibrosis by activating a metastatic cancer signature in invasive lung fibroblasts The Journal of experimental medicine 10/3/2022 [PMID: 35980387]
K Kishimoto, K Iwasawa, A Sorel, C Ferran-Her, L Han, M Morimoto, JM Wells, T Takebe, AM Zorn Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems Nature Protocols, 2022-08-17;0(0):. 8/17/2022 [PMID: 35978039]
Emily E. Fink, Surbhi Sona, Uyen Tran, Pierre-Emmanuel Desprez, Matthew Bradley, Hong Qiu et al. Single-cell and spatial mapping Identify cell types and signaling Networks in the human ureter Developmental Cell 2022-08-01 [PMID: 35914526]
Guojie Zhong, Priyanka Ahimaz, Nicole A. Edwards, Jacob J. Hagen, Christophe Faure, Qiao Lu et al. Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas Human Genetics and Genomics Advances 2022-07-14 [PMID: 35519826]
Show All 29 Publications.

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Bioinformatics

Gene Symbol FOXF1
Uniprot