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HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

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Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4 ug/ml anti-hFOXA1 (3B11NB) in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Whole cell protein from PC3 (1) and MCF7 (2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Immunocytochemistry/ Immunofluorescence: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - FOXA1 (3B11NB) antibody was tested in HepG2 cells at a 1:25 dilution against DyLight 488 (Green) using a 40X objective. Actin ...read more
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human prostate.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of LnCAP cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with ...read more
Western Blot: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in partial recombinant FOXA1 protein and PC3 lysate, respectivetly.
Immunohistochemistry-Paraffin: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis of FOXA1 (3B11NB) in human breast adenocarcinoma.
Flow Cytometry: HNF-3 alpha/FoxA1 Antibody (3B11NB) [NBP2-45269] - Analysis using the Azide Free version of NBP2-45269. Staining of HepG2 cells (1 x 10^6 cells/ml) with FOXA1 antibody, clone 3B11NB (orange), shown with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
3B11NB
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-45269AF350
Alexa Fluor 405
NBP2-45269AF405
Alexa Fluor 488
NBP2-45269AF488
Alexa Fluor 532
NBP2-45269AF532
Alexa Fluor 594
NBP2-45269AF594
Alexa Fluor 647
NBP2-45269AF647
Alexa Fluor 700
NBP2-45269AF700
Alexa Fluor 750
NBP2-45269AF750
Allophycocyanin
NBP2-45269APC
Biotin
NBP2-45269B
DyLight 350
NBP2-45269UV
DyLight 405
NBP2-45269V
DyLight 488
NBP2-45269G
DyLight 550
NBP2-45269R
DyLight 594
NBP2-45269DL594
DyLight 650
NBP2-45269C
DyLight 680
NBP2-45269FR
DyLight 755
NBP2-45269IR
FITC
NBP2-45269F
HRP
NBP2-45269H
Janelia Fluor 549
NBP2-45269JF549
Janelia Fluor 646
NBP2-45269JF646
PE
NBP2-45269PE
PE/Cy7
NBP2-45269PECY7
PerCP
NBP2-45269PCP
mFluor Violet 450 SE
NBP2-45269MFV450
mFluor Violet 500 SE
NBP2-45269MFV500
mFluor Violet 610 SE
NBP2-45269MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-45269SS
Azide and BSA Free
NBP2-45270SS
Azide and BSA Free
NBP2-45270
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HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free Summary

Immunogen
Partial recombinant human FOXA1 protein (between amino acids 350-500) [Uniprot: P55317].
Predicted Species
Mouse (98%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 25 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:25 - 1:50
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml
Publications
Read Publications using
NBP2-45269 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free

  • forkhead box A1
  • Forkhead box protein A1
  • FoxA1
  • hepatocyte nuclear factor 3, alpha
  • hepatocyte nuclear factor 3-alpha
  • HNF3 alpha
  • HNF-3 alpha
  • HNF-3A
  • HNF-3-alpha
  • HNF3ATCF-3A
  • MGC33105
  • TCF3A
  • Transcription factor 3A

Background

FoxA1 (forkhead box A1), also called HNF-3a (hepatocyte nuclear factor 3 alpha) or TCF3A (transcription factor 3A) is a 473 amino acid (aa), 49 kDa (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93% and 86% aa identity with mouse and rat FoxA1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-45269
HNF-3 alpha/FoxA1 Antibody (3B11NB) - BSA Free
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC

Publications for HNF-3 alpha/FoxA1 Antibody (NBP2-45269)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF/IHC, IHC-P.


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(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol FOXA1
Uniprot