Novus Biologicals products are now on bio-techne.com

FLJ21908 Antibody

Images

 
Independent Antibodies: Western Blot: FLJ21908 Antibody [NBP1-89850] - Analysis using Anti-RPAP3 antibody NBP1-89850 (A) shows similar pattern to independent antibody NBP1-89849 (B).
Immunocytochemistry/ Immunofluorescence: FLJ21908 Antibody [NBP1-89850] - Staining of human cell line U-2 OS shows positivity in cytoplasm.
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human cerebral cortex.
Western Blot: FLJ21908 Antibody [NBP1-89850] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Genetic Strategies: Western Blot: FLJ21908 Antibody [NBP1-89850] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading ...read more
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Independent Antibodies: Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human cerebral cortex, colon, fallopian tube and testis using Anti-RPAP3 antibody NBP1-89850 (A) shows similar ...read more
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human testis.
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human fallopian tube.
Immunohistochemistry-Paraffin: FLJ21908 Antibody [NBP1-89850] - Staining of human colon.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

FLJ21908 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SEKMPIEIEQKPAQFATTVLPPIPANSFQLESDFRQLKSSPDMLYQYLKQIEPSLYPKLFQKNLDPDVFNQIVKILHDFYIEKEKPLLIFEILQRLSELKRFDMAVMFM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
FLJ21908 Protein (NBP1-89850PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for FLJ21908 Antibody

  • FLJ21908
  • RNA polymerase II associated protein 3
  • RNA polymerase II-associated protein 3
  • spag

Background

FLJ21908, also known as RNA polymerase II associated protein, has a 665 amino acid isoform that is 76 kDa, a 631 amino acid isoform that is 72 kDa, and a 506 amino acid isoform that is 57 kDa; is involved in formation of an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Disease research suggests correlation of this protein with sickle cell trait and malaria. This protein has interactions with HSP90AA1, HSP90AB1, MAP3K3, ITCH, PPP1CA, POLR2A, HELLS, WDR92 and TEL02.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-40354
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
NBP1-92594
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-26504
Species: Hu, Mu, Rt
Applications: B/N, Func-Inh, In vitro, In vivo
AF2288
Species: Hu
Applications: ICC, IHC, Simple Western, WB
NBP1-92269
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-32016
Species: Hu
Applications: IHC, IHC-P
AF640
Species: Mu
Applications: IHC, WB
NBP1-80694
Species: Hu
Applications: ICC/IF, WB
NBP1-80817
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-13075
Species: Ba, Hu, Pm, Mu, Rt
Applications: DB, ELISA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
H00008086-M02
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-81761
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-308
Species: Hu, Mu
Applications: Func, ICC/IF, IP, In vitro, KO, WB
H00023049-M02
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
DLP00
Species: Hu
Applications: ELISA
NBP2-14512
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-89850
Species: Hu
Applications: WB, ICC/IF, IHC, KD

Publications for FLJ21908 Antibody (NBP1-89850) (0)

There are no publications for FLJ21908 Antibody (NBP1-89850).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for FLJ21908 Antibody (NBP1-89850) (0)

There are no reviews for FLJ21908 Antibody (NBP1-89850). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FLJ21908 Antibody (NBP1-89850) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our FLJ21908 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol RPAP3