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FIP1/RCP Antibody

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Biological Strategies: Western Blot: FIP1/RCP Antibody [NBP2-37699] - Whole cell lysate (50ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody used ...read more
Immunoprecipitation: FIP1/RCP Antibody [NBP2-37699] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-RCP antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

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FIP1/RCP Antibody Summary

Immunogen
This antibody maps to a region between residue 1233 to 1283 of human RAB11 Coupling Protein using the numbering given in entry NP_001002814.2 (GeneID 80223).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB11FIP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FIP1/RCP Antibody

  • DKFZp686E2214
  • EC 3.2.1.28
  • EC 6.1.1.7
  • FLJ22524
  • FLJ22622
  • MGC78448
  • NOEL1A
  • RAB11 coupling protein
  • RAB11 family interacting protein 1 (class I)
  • rab11 family-interacting protein 1
  • Rab11-FIP1
  • Rab-coupling protein
  • Rab-interacting recycling protein
  • RCPRab effector protein

Background

Proteins of the large Rab GTPase family have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP1 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RAB11FIP1
Uniprot