![Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1561) - Azide and BSA Free [NBP3-08382] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561).](http://images.novusbio.com/fullsize/Filaggrin-Antibody-rFLG-1561-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08382-img0001.jpg "Immunohistochemistry-Paraffin: Filaggrin Antibody (rFLG/1561) - Azide and BSA Free [NBP3-08382] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | rFLG/1561 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07377). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary) (Uniprot: P20930) |
| Localization | Cytoplasmic |
| Marker | Keratinocyte Differentiation Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FLG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | FLG |
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