![Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Monoclonal Antibody (FLG/1561).](http://images.novusbio.com/fullsize/Filaggrin-Antibody-FLG-1561-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54366-img0002.jpg "Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Monoclonal Antibody (FLG/1561).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | FLG/1561 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53243). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary) (Uniprot: P20930) |
| Localization | Cytoplasmic |
| Marker | Keratinocyte Differentiation Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FLG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Use in Western Blot reported in scientific literature (PMID: 32102205). Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Publications |
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| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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| Publication using NBP2-54366 | Applications | Species |
|---|---|---|
| Jahanshahi M Engineered infected epidermis model for in vitro study of the skin proinflammatory response Micromachines (Basel) 2020-02-28 [PMID: 32102205] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Filaggrin Antibody (NBP2-54366)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FLG |
![Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Analysis of Protein Array containing >19,000 full-length human proteins using Filaggrin Antibody (FLG/1561) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Filaggrin-Antibody-FLG-1561-Azide-and-BSA-Free-Protein-Array-NBP2-54366-img0003.jpg "Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Analysis of Protein Array containing >19,000 full-length human proteins using Filaggrin Antibody (FLG/1561) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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