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CREM Antibody (3B5)

Images

 
Western Blot: CREM Antibody (3B5) [H00001390-M02] - CREM monoclonal antibody (M02), clone 3B5. Analysis of CREM expression in human liver.
Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, stained using CREM antibody at 1:200. Secondary Antibody: Alexa ...read more
Immunocytochemistry/ Immunofluorescence: CREM Antibody (3B5) [H00001390-M02] - Analysis of monoclonal antibody to CREM on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: CREM Antibody (3B5) [H00001390-M02] - Detection limit for recombinant GST tagged CREM is 0.03 ng/ml as a capture antibody.
Genetic Strategies: Knockdown Validated: CREM Antibody (3B5) [H00001390-M02] - UTGB1, primary glioblastoma cell line, expressing the EWSR1-CREM fusion, treated with non-target siRNA and different concentrations ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, KD
Clone
3B5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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CREM Antibody (3B5) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
CREM (NP_853549, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY
Specificity
CREM (3B5)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CREM
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 2 Reviews rated 4.5
using
H00001390-M02 in the following applications:

Publications
Read Publication using H00001390-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CREM Antibody (3B5)

  • cAMP response element modulator
  • cAMP responsive element modulator
  • cAMP-responsive element modulator
  • hCREM 2alpha-b protein
  • hCREM 2beta-a protein
  • hCREM-2
  • ICER
  • inducible cAMP early repressor ICER
  • Inducible cAMP early repressor
  • MGC111110
  • MGC17881
  • MGC41893

Background

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CREM Antibody (H00001390-M02)(1)


Showing Publication 1 - 1 of 1.
Publication using H00001390-M02 Applications Species
David K, Isis P Dual regulation of miR-375 and CREM genes in pancreatic beta cells. Islets. 2022-04-04 [PMID: 35377267]

Reviews for CREM Antibody (H00001390-M02) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using H00001390-M02:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry CREM H00001390-M02
Enlarge
4
reviewed by:
Vanina D. Heuser
ICC Human 08/30/2018
View

Summary

ApplicationImmunocytochemistry
Sample Testedhuman glioblastoma,Primary Glioblastoma cell line UTGB1
SpeciesHuman
Lot10061-3B5
Western Blot CREM H00001390-M02
Enlarge
5
reviewed by:
Vanina D. Heuser
WB Human 08/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedPrimary Glioblastoma cell line UTGB1
SpeciesHuman
Lot10061-3B5

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CREM Antibody (H00001390-M02) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional CREM Products

Research Areas for CREM Antibody (H00001390-M02)

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Blogs on CREM

There are no specific blogs for CREM, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Contact Information

Product PDFs

Calculators

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Vanina D. Heuser
08/30/2018
Application: ICC
Species: Human

Vanina D. Heuser
08/30/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol CREM
Entrez
OMIM
Uniprot