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Blk Antibody (7A12)

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Western Blot: Blk Antibody (7A12) [H00000640-M02] - Analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M02), clone 7A12.Lane 1: BLK transfected lysate (Predicted MW: 57.7 KDa).Lane 2: ...read more
Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in PC-12.
Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in Raw 264.7.
ELISA: Blk Antibody (7A12) [H00000640-M02] - Detection limit for recombinant GST tagged BLK is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow
Clone
7A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Blk Antibody (7A12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
BLK (AAH07371, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKG
Localization
Nuclear
Specificity
BLK - B lymphoid tyrosine kinase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BLK
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. FACS usage was reported in scientific literature.
Reviewed Applications
Read 1 Review rated 4
using
H00000640-M02 in the following applications:

Publications
Read Publications using
H00000640-M02 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Blk Antibody (7A12)

  • B lymphocyte kinase
  • B lymphoid tyrosine kinase
  • BLK nonreceptor tyrosine kinase
  • Blk
  • EC 2.7.10.2
  • MGC10442
  • MODY11
  • p55-Blk
  • tyrosine-protein kinase Blk

Background

The gene encoding BLK is a member of the SRC family of protooncogenes and shows preferential expression in B-lymphoid cells. It is thought to function in a signal transductory pathway specific to this lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Blk Antibody (H00000640-M02)(4)

Review for Blk Antibody (H00000640-M02) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00000640-M02:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Blk H00000640-M02
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4
reviewed by:
Verified Customer
WB Human 05/16/2011
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Summary

ApplicationWestern Blot
Sample Tested100ng recombinant BLK, rLCK, rLYN, rFYN. 3ug Ramos whole cell lysate. 20ug Jurkat whole cell lysate.
SpeciesHuman
Lot6342-7A12-00AaY6

Product General Protocols

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WB Video Protocol

FAQs for Blk Antibody (H00000640-M02). (Showing 1 - 1 of 1 FAQs).

  1. What is the concentration of H00000640-M02 Lot E9231-7A12?
    • The concentration of Lot# Lot E9231-7A12 is 0.5mg/mL.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
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1
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Verified Customer
05/16/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol BLK
Entrez
OMIM
Uniprot