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Recombinant Human FGF acidic/FGF1 Protein

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SDS-Page: Human FGF acidic/FGF1 Protein [NBP2-35052]
SDS-Page: Human FGF acidic/FGF1 Protein [NBP2-35052]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier-Free
NBP2-35052-1mg
Carrier-Free
NBP2-35052-100ug
Carrier-Free
NBP2-35052-500ug
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Recombinant Human FGF acidic/FGF1 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 141 amino acids corresponding to FGF acidic/FGF1 Source: E. coli

Uniprot ID: P05230

Amino Acid Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Details of Functionality
FGF acidic/FGF1 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0 x 10^6 IU/mg in the presence of 10 ug/ml of heparin.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF1
Purity
>95%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 1 EU/ug of FGF acidic/FGF1 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
16.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-35052 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human FGF acidic/FGF1 Protein

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Background

Human FGF1 (aFGF), encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human FGF1 (aFGF) shares 54 % amino acid sequence identity with bFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types "b", "c". FGF1 (aFGF) can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF1 (aFGF) are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Review for FGF acidic/FGF1 Protein (NBP2-35052) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-35052:
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Enzymatic activity in vitro FGF acidic/FGF1 NBP2-35052
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Verified Customer
 Enzymatic activity in vitro 08/04/2017
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Summary

ApplicationEnzymatic activity in vitro
LotH10401011

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Enzymatic activity in vitro FGF acidic/FGF1 NBP2-35052
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Application: Enzymatic activity in vitro
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Bioinformatics

Gene Symbol FGF1
Uniprot