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Recombinant Mouse FGF-17 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

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Recombinant Mouse FGF-17 Protein Summary

Description
A single non-glycosylated polypeptide chain containing 194 amino acids corresponding to FGF-17 Source: E. coli

Amino Acid Sequence: TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT
Details of Functionality
FGF-17 protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg in the presence of 10 ug/ml of heparin.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FGF17
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of FGF-17 as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.
Preservative
No Preservative
Concentration
LYOPH
Purity
>98%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile PBS to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Mouse FGF-17 Protein

  • FGF-13
  • FGF17
  • FGF-17
  • fibroblast growth factor 17

Background

The FGF17 gene codes for a fibroblast growth factor 17 protein that in isoform 1 is 216 amino acids long at 24 kDA and in isoform 2 is 205 amino acids long at 23 kDA. This gene is a member of the fibroblast growth factor (FGF) family so is thought to participate in nervous system development and function, specifically in embryonic development as well as a signaling molecule in the induction and patterning of the embryonic brain. FGF14 is involved in regulation of actin cytoskeleton, MAPK signaling, signaling by FGFR and FGFR mutants, as well as downstream signal transduction. It interacts with genes EGFR, FGFR1, FGFR2, FGFR3, and FGFR4. FGF14 is associated with pancreatitis, breast cancer, leukemia, melanoma, and adenocarcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol FGF17