| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Simple Western, KO |
| Clone | 647113 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human Fatty Acid Synthase/FASN Ser10-Cys212 Accession # P49327 |
| Specificity | Detects human and mouse Fatty Acid Synthase/FASN in Western blots. |
| Source | N/A |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FASN |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration. |
FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.
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| Publication using MAB5927 | Applications | Species |
|---|---|---|
| Chuang, HJ;Chiu, L;Liao, BJ;Chang, CY;Wu, GC;Tseng, YC;Chou, MY;Hwang, PP; Environmental acidification drives inter-organ energy mobilization to enhance reproductive performance in medaka (Oryzias latipes) Journal of hazardous materials 2024-10-18 [PMID: 39442299] (Western Blot, Fish - Oryzias latipes (Medaka or Japanese Rice Fish)) | Western Blot | Fish - Oryzias latipes (Medaka or Japanese Rice Fish) |
Secondary Antibodies |
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