![Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Fatty Acid Synthase Antibody (325) detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase Antibody (325) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Immunocytochemistry-Immunofluorescence-NBP2-43822-img0005.jpg "Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Fatty Acid Synthase Antibody (325) detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Fatty Acid Synthase stained by Fatty Acid Synthase Antibody (325) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar= 10um.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clone | 325 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase/FASN. The exact sequence is proprietary. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FASN |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
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| Publication using NBP2-43822 | Applications | Species |
|---|---|---|
| GOnczi M, Csemer A, SzabO L et al. Astaxanthin Exerts Anabolic Effects via Pleiotropic Modulation of the Excitable Tissue International journal of molecular sciences 2022-01-14 [PMID: 35055102] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for Fatty Acid Synthase/FASN Antibody (NBP2-43822)Find related products by research area.
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| Gene Symbol | FASN |
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0002.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:500")
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000.](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0003.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE Fatty Acid Synthase antibody dilution: 1:5000.")
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325].](http://images.novusbio.com/fullsize/Fatty-Acid-Synthase-FASN-Antibody-325-Western-Blot-NBP2-43822-img0004.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [GT325].")
![Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase/FASN antibody [GT325] (NBP2-43822) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)](http://images.novusbio.com/fullsize/nbp2-43822_mouse-monoclonal-fatty-acid-synthase-fasn-antibody-325-910202419593552.jpg "Western Blot: Fatty Acid Synthase/FASN Antibody (325) [NBP2-43822] - Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase/FASN antibody [GT325] (NBP2-43822) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)")
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