![Western Blot: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Fascin-Antibody-Knockdown-Validated-NBP1-91887-img0021.jpg "Western Blot: Fascin Antibody [NBP1-91887] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout (KO) HeLa cell line. PVDF membrane was probed with 0.4 ug/ml of Rabbit Anti-Human Fascin Polyclonal Antibody (Catalog # NBP1-91887) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: GKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQ |
| Marker | Dendritic Cell Marker |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FSCN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Fascin Antibody (NBP1-91887)Find related products by research area.
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| Gene Symbol | FSCN1 |
![Western Blot: Fascin Antibody [NBP1-91887] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-FSCN1 antibody. Corresponding FSCN1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/Fascin-Antibody-Western-Blot-NBP1-91887-img0014.jpg "Western Blot: Fascin Antibody [NBP1-91887] - Analysis in human cell lines PC-3 and SK-MEL-30 using anti-FSCN1 antibody. Corresponding FSCN1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP1-91887 (A) shows similar protein distribution across tissues to independent antibody NBP2-58042 (B).](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0018.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum, cerebral cortex, testis and tonsil using Anti-Fascin antibody NBP1-91887 (A) shows similar protein distribution across tissues to independent antibody NBP2-58042 (B).")
![Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP1-91887] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91887-img0010.jpg "Immunocytochemistry/Immunofluorescence: Fascin Antibody [NBP1-91887] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-NBP1-91887-img0020.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.")
![Western Blot: Fascin Antibody [NBP1-91887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Fascin-Antibody-Western-Blot-NBP1-91887-img0008.jpg "Western Blot: Fascin Antibody [NBP1-91887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0015.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0017.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.")
![Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Fascin-Antibody-Immunohistochemistry-Paraffin-NBP1-91887-img0019.jpg "Immunohistochemistry-Paraffin: Fascin Antibody [NBP1-91887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/TNF_RII_DRT200_ELISA_176.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_817.jpg)
![N/A TNF RII/TNFRSF1B [HRP]](https://images.novusbio.com/images2/DATA_TNF_RII_DRT200_ELISA_818.jpg)
![Western Blot LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12543.jpg)
![Immunocytochemistry LEDGF Antibody (762815) [Unconjugated]](https://images.novusbio.com/images2/12613.jpg)
![Neutralization Siglec-7/CD328 Antibody [Unconjugated]](https://images.novusbio.com/images2/Siglec-7_AF1138_Block_Neutralize_8760.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A TNF RI/TNFRSF1A [HRP]](https://images.novusbio.com/images2/DATA_TNF_RI_DRT100_ELISA_815.jpg)
![N/A TNF RI/TNFRSF1A [HRP]](https://images.novusbio.com/images2/DATA_TNF_RI_DRT100_ELISA_816.jpg)
![N/A TNF RI/TNFRSF1A [HRP]](https://images.novusbio.com/images2/TNF_RI_DRT100_ELISA_175.jpg)
![Bioactivity IL-4 [Unconjugated]](https://images.novusbio.com/images/protein/6507-ilcf_recombinant-human-il-4-cho-expressed-protein-cf-bioactivity-272020133214.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![SDS-PAGE IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_304.jpg)
![Bioactivity IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images2/IL-1_beta_201-LB_306.jpg)
![SEC-MALS IL-1 beta/IL-1F2 [Unconjugated]](https://images.novusbio.com/images/201-lb_recombinant-human-il-1-beta-il-1f2-protein-sec-mals-123202491556.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")