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F4/80 Antibody (521204) [Phycoerythrin]

 (6 Publications)
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RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5580P, filled histogram) or isotype control antibody (Catalog # IC006P, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
521204
Clonality
Monoclonal
Host
Rat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5580U-100UG
Alexa Fluor 405
FAB5580V-100UG
Alexa Fluor 488
FAB5580G-100UG
Alexa Fluor 532
FAB5580X
Alexa Fluor 594
FAB5580T-100UG
Alexa Fluor 647
FAB5580R-100UG
Alexa Fluor 700
FAB5580N-100UG
Alexa Fluor 750
FAB5580S-100UG
Allophycocyanin
FAB5580A-100UG
Allophycocyanin/Cy7
FAB5580APCCY7
Biotin
FAB5580B
CoraFluor 1
FAB5580CL1
DyLight 350
FAB5580D
DyLight 405
FAB5580E
DyLight 488
FAB5580K
DyLight 550
FAB5580L
DyLight 594
FAB5580M
DyLight 650
FAB5580W
DyLight 680
FAB5580Y
DyLight 755
FAB5580Z
FITC
FAB5580F
HRP
FAB5580H
Janelia Fluor 525
FAB5580JF525
Janelia Fluor 549
FAB5580I
Janelia Fluor 646
FAB5580J
Janelia Fluor 669
FAB5580JF669
PE/Atto594
FAB5580PEATT594
PE/Cy5.5
FAB5580PECY55
PE/Cy7
FAB5580PECY7
Unconjugated
MAB5580
mFluor Violet 450 SE
FAB5580MFV450
mFluor Violet 500 SE
FAB5580MFV500
mFluor Violet 610 SE
FAB5580MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB5580
Carrier Free
MAB5580
Carrier Free
MAB5580-SP
Carrier Free
FAB5580V-100UG
Carrier Free
FAB5580U-100UG
Carrier Free
FAB5580T-100UG
Carrier Free
FAB5580S-100UG
Carrier Free
FAB5580R-100UG
Carrier Free
FAB5580N-100UG
Carrier Free
FAB5580G-100UG
Carrier Free
FAB5580A-100UG
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F4/80 Antibody (521204) [Phycoerythrin] Summary

Immunogen
NS0 mouse myeloma cell line transfected with mouse F4/80/EMR1
Gln28-Gly931
Accession # Q61549
Specificity
Detects mouse F4/80/EMR1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
EMR1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Publications
Read Publications using
FAB5580P in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for F4/80 Antibody (521204) [Phycoerythrin]

  • ADGRE1
  • cell surface glycoprotein F4/80
  • DD7A5-
  • DD7A5-7
  • EGF-like module containing, mucin-like, hormone receptor-like sequence 1
  • EGF-like module receptor 1
  • EGF-like module-containing mucin-like hormone receptor-like 1
  • EGF-TM7
  • Emr
  • EMR1 hormone receptor
  • Emr1
  • F4/80
  • Gpf480
  • Ly7
  • Ly71
  • lymphocyte antigen 71
  • Mouse EMR1
  • Rat EMR1
  • TM7L
  • TM7LN3

Background

F4/80, also known as EMR1 in humans, is a 160 kDa glycosylated protein that belongs to the EGF-TM7 family of adhesion molecules. It is expressed on splenic red pulp and bone marrow, macrophages, microglia, Langerhans cells, monocytes, eosinophils, and CD116+ dendritic cells in mouse, but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance, being necessary for the development of CD8+ T regs. It also serves as a marker for IL-1 beta + proinflammatory macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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F4/80 Antibody
(521204) [Phycoerythr
FAB5580P
F4/80 Antibody (521204) [Phycoerythrin]
Species: Mu
Applications: Flow

Publications for F4/80 Antibody (FAB5580P)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow Cytometry, ICC.


Filter By Application
Flow Cytometry
(4)
ICC
(1)
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Mouse
(5)
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Showing Publications 1 - 6 of 6.
Publications using FAB5580P Applications Species
JM Motta, VM Rumjanek, A Mantovani, M Locati Tumor-Released Products Promote Bone Marrow-Derived Macrophage Survival and Proliferation Biomedicines, 2021-10-04;9(10):. 2021-10-04 [PMID: 34680504] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Xu, C Ge, Y Qin, D Lou, Q Li, J Feng, Y Wu, L Hu, B Wang, J Tan Functional loss of inactive rhomboid-like protein�2 mitigates obesity by suppressing pro-inflammatory macrophage activation-triggered adipose inflammation Mol Metab, 2020-01-22;34(0):112-123. 2020-01-22 [PMID: 32180551] (ICC, Mouse) ICC Mouse
J Ben, B Jiang, D Wang, Q Liu, Y Zhang, Y Qi, X Tong, L Chen, X Liu, Y Zhang, X Zhu, X Li, H Zhang, H Bai, Q Yang, J Ma, EAC Wiemer, Y Xu, Q Chen Major vault protein suppresses obesity and atherosclerosis through inhibiting IKK-NF-?B signaling mediated inflammation Nat Commun, 2019-04-17;10(1):1801. 2019-04-17 [PMID: 30996248] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Heim C, Vidlak D, Scherr T, Hartman C, Garvin K, Kielian T IL-12 promotes myeloid-derived suppressor cell recruitment and bacterial persistence during Staphylococcus aureus orthopedic implant infection. J Immunol, 2015-03-11;194(8):3861-72. 2015-03-11 [PMID: 25762781] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Fermino M, Dias F, Lopes C, Souza M, Cruz A, Liu F, Chammas R, Roque-Barreira M, Rabinovich G, Bernardes E Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection. Eur J Immunol, 2013-05-17;43(7):1806-17. 2013-05-17 [PMID: 23592449] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Yun Wang, Qing Jiang Gamma-tocotrienol inhibits lipopolysaccharide-induced interlukin-6 and granulocyte-colony stimulating factor by suppressing C/EBP-beta and NF-kappa B in macrophages The Journal of Nutritional Biochemistry 6/1/2013 [PMID: 23246159]

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Product General Protocols

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FAQs for F4/80 Antibody (FAB5580P). (Showing 1 - 4 of 4 FAQs).

  1. Are the F4/80/EMR1 antibodies validated in Simple Western?
    • No, F4/80/EMR1 antibodies are not tested in Simple Western at this moment.
  2. Does F4/80/EMR1 antibodies come in lyophilized form?
    • Yes, we carry 2 F4/80 antibodies in lyophilized form: MAB5580 and NBP1-06975
  3. What is the theoretical molecular weight for F4/80/EMR1 antibodies?
    • The TMW of F4/80/EMR1 antibodies is approximately 160 kDa.
  4. What is the immunogen sequence of this F4/80/EMR1 antibodies antibody?
    • A sequence corresponding to Gln28 - Gly931 of mouse F4/80/EMR1.

Secondary Antibodies

 

Isotype Controls

Additional F4/80 Products

Array FAB5580P

Blogs on F4/80.

Check out the latest blog posts on F4/80.
TMEM 119 is a specific marker of microglia cells
By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse F4/80/EMR1 Monoclonal Antibody (Catalog # MAB5580, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
F4/80 Antibody (521204) [Unconjugated]
MAB5580
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
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Bioinformatics

Gene Symbol EMR1
Uniprot