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RPL22 Antibody

Images

 
Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for ...read more
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
b) Polysome profile of U251 cells untreated (NT) or puromycin treated (Puro), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RPL22 Antibody Summary

Immunogen
Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPL22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/mL
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:16000
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06069 in the following applications:

Publications
Read Publications using
NBP1-06069 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26962690).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPL22 Antibody

  • EAPHBP15
  • EBER-associated protein
  • Epstein-Barr virus small RNA-associated protein
  • Epstein-Barr-encoded RNA-associated protein
  • HBP15/L22
  • heparin-binding protein 15
  • Heparin-binding protein HBp15
  • ribosomal protein L22,60S ribosomal protein L22

Background

RPL22, also known as 60S ribosomal protein L22, is a 15kDa 128 amino acid protein. The primary function of RPL22 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL22 in relation to hepatitis, carcinoma, malaria, leukemia, hepatitis c, pneumonia and lung carcinoma. RPL22 has been linked to the virulence, methylation, contact inhibition, pathogenesis and cell growth pathways where it interacts with SHC1, UBA5, CSE1L, PRKAG1 and MAP3K14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPL22 Antibody (NBP1-06069)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC-P
(1)
WB
(5)
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Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-06069 Applications Species
Luan y, Tang N, yang J et al. Deficiency of ribosomal proteins reshapes the transcriptional and translational landscape in human cells Nucleic acids research 2022-02-08 [PMID: 35137207] (WB, Human) WB Human
Bogdanov V, Soltisz AM, Moise N Et al. Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes Basic research in cardiology 2021-10-28 [PMID: 34713358] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse
Herviou P, Le Bras M, Dumas L et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma Nat Commun 2020-05-27 [PMID: 32461552] (WB, Human) WB Human
Fu K, Tian S, Tan H Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol. 2019-05-14 [PMID: 31088452] (WB, Mouse) WB Mouse
Li L, Wang M, Wang M et al. A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells Cell Death Dis 2016-03-11 [PMID: 26962690] (WB, Mouse) WB Mouse
Lin SJ, Chang KP, Hsu CW et al. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics 2013-09-27 [PMID: 24080422] (WB, IHC-P, Human) WB, IHC-P Human
Jin J, Jing W, Lei X-X et al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res. 2011-01-01 [PMID: 21247876]
Anderson SJ, Lauritsen JP, Hartman MG et al. Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint. Immunity 2007-06-01 [PMID: 17555992]

Review for RPL22 Antibody (NBP1-06069) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-06069:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot RPL22 NBP1-06069
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4
reviewed by:
Verified Customer
WB Human 11/02/2020
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Summary

ApplicationWestern Blot
Sample TestedHEK293 cell lysate
SpeciesHuman
LotP1 E071019

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FAQs for RPL22 Antibody (NBP1-06069) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RPL22 Products

Array NBP1-06069

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Blogs on RPL22

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Verified Customer
11/02/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol RPL22
Entrez
Uniprot