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SAP155 Antibody (4A3G9)

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Western Blot: SAP155 Antibody (4A3G9) [NBP3-33555] - Western blot analysis of various lysates using SAP155 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry: SAP155 Antibody (4A3G9) [NBP3-33555] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using SAP155 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure ...read more
Immunohistochemistry: SAP155 Antibody (4A3G9) [NBP3-33555] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using SAP155 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen ...read more
Immunohistochemistry: SAP155 Antibody (4A3G9) [NBP3-33555] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using SAP155 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen ...read more
Immunohistochemistry: SAP155 Antibody (4A3G9) [NBP3-33555] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SAP155 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4A3G9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SAP155 Antibody (4A3G9) Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SAP155 (O75533).

Sequence:
MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQ
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SF3B1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
Theoretical MW
146 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SAP155 Antibody (4A3G9)

  • Hsh155
  • pre-mRNA processing 10
  • pre-mRNA splicing factor SF3b, 155 kDa subunit
  • Pre-mRNA-splicing factor SF3b 155 kDa subunit
  • Prp10
  • PRPF10
  • SAP 155
  • SAP155splicing factor 3b, subunit 1, 155kD
  • SF3B155
  • SF3b155PRP10
  • spliceosome associated protein 155
  • Spliceosome-associated protein 155
  • splicing factor 3B subunit 1
  • splicing factor 3b, subunit 1, 155kDa

Background

SF3B155 is a subunit of the U2 small nuclear ribonucleoproteins complex (U2 snRNP) that functions in pre-mRNA splicing. SF3B155 together with splicing factor 3a binds pre-mRNA upstream of the branchpoint sequence to anchor U2 snRNP to the pre-mRNA. SF3B155 is also a component of the U12-type splicesome that is involved in the splicing of a rare class of nuclear pre-mRNA introns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SF3B1