![Knockout Validated: ERK2 Antibody (SZ25-01) [NBP2-67360] - Lysates of HeLa WT and MAPK1 KO were prepared, and 30 ug of protein were processed for immunoblot with the indicated Mitogen-activated protein kinase 1 antibodies. The Ponceau stained transfers of each blot are shown. Antibody dilution used: 1/1000. Predicted band size: 41 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Knockout-Validated-NBP2-67360-img0011.jpg "Knockout Validated: ERK2 Antibody (SZ25-01) [NBP2-67360] - Lysates of HeLa WT and MAPK1 KO were prepared, and 30 ug of protein were processed for immunoblot with the indicated Mitogen-activated protein kinase 1 antibodies. The Ponceau stained transfers of each blot are shown. Antibody dilution used: 1/1000. Predicted band size: 41 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human ERK2 aa 311-360 / 360. (SwissProt: P28482 Human; SwissProt: P63085 Mouse; SwissProt: P63086 Rat) |
| Localization | Cytoplasm, Nucleus, Cytoskeleton, Membrane, Cell junction. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | MAPK1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-P | Mouse | 01/01/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | MAPK1 |
![Knockout Validated: ERK2 Antibody (SZ25-01) [NBP2-67360] - HeLa WT and MAPK1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with the indicated Mitogen-activated protein kinase 1 antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the red (grayscale) channels are shown.WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Bars = 10 um. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Knockout-Validated-NBP2-67360-img0012.jpg "Knockout Validated: ERK2 Antibody (SZ25-01) [NBP2-67360] - HeLa WT and MAPK1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with the indicated Mitogen-activated protein kinase 1 antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the red (grayscale) channels are shown.WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Bars = 10 um. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Western-Blot-NBP2-67360-img0009.jpg "Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0004.jpg "Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse intestine tissue using anti-ERK2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunohistochemistry-Paraffin-NBP2-67360-img0010.jpg "Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse intestine tissue using anti-ERK2 antibody. Image from verified customer review.")
![Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Flow-Cytometry-NBP2-67360-img0001.jpg "Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Western-Blot-NBP2-67360-img0008.jpg "Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12")
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0002.jpg "Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunocytochemistry-Immunofluorescence-NBP2-67360-img0003.jpg "Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunohistochemistry-Paraffin-NBP2-67360-img0005.jpg "Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse pancreas tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunohistochemistry-Paraffin-NBP2-67360-img0006.jpg "Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse pancreas tissue using anti-ERK2 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/ERK2-Antibody-SZ25-01-Immunohistochemistry-Paraffin-NBP2-67360-img0007.jpg "Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.")
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