![Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NB100-2305-img0005.jpg "Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Rt, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474) |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LIMA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC reactivity reported in (PMID: 21625216). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a') In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b',b''). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b'', asterisk). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/578), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2305-img0007.jpg "Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a') In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b',b''). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b'', asterisk). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/578), licensed under a CC-BY license.")
![Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunoprecipitation-NB100-2305-img0006.jpg "Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")