![Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NB100-2305-img0005.jpg "Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Rt, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474) |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LIMA1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC reactivity reported in (PMID: 21625216). |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a') In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b',b''). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b'', asterisk). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/578), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2305-img0007.jpg "Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a') In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b',b''). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b'', asterisk). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/578), licensed under a CC-BY license.")
![Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunoprecipitation-NB100-2305-img0006.jpg "Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2305_rabbit-polyclonal-eplin-antibody-31020241683934.jpg "Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2305_rabbit-polyclonal-eplin-antibody-31020241552527.jpg "Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")