![Western Blot: Supervillin Antibody [NBP1-90363] - Analysis in human cell lines A-431 and PC-3 using anti-SVIL antibody. Corresponding SVIL RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Supervillin-Antibody-Western-Blot-NBP1-90363-img0012.jpg "Western Blot: Supervillin Antibody [NBP1-90363] - Analysis in human cell lines A-431 and PC-3 using anti-SVIL antibody. Corresponding SVIL RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: NARYQTQPVTLGEVEQVQSGKLIPFSPAVNTSVSTVASTVAPMYAGDLRTKPPLDHNASATDYKFSSSIENSDSPVRSILKSQAWQPLVEGSENKGMLREYGETESKRALTGRDSGMEKYGSFEEAEASYPILNRARE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SVIL |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SVIL |
![Immunocytochemistry/Immunofluorescence: Supervillin Antibody [NBP1-90363] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Supervillin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90363-img0007.jpg "Immunocytochemistry/Immunofluorescence: Supervillin Antibody [NBP1-90363] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Supervillin-Antibody-Immunohistochemistry-Paraffin-NBP1-90363-img0017.jpg "Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.")
![Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Supervillin-Antibody-Immunohistochemistry-Paraffin-NBP1-90363-img0014.jpg "Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.")
![Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human prostate shows moderate to strong cytoplasmic positivity in smooth muscle cells](http://images.novusbio.com/fullsize/Supervillin-Antibody-Immunohistochemistry-Paraffin-NBP1-90363-img0015.jpg "Immunohistochemistry-Paraffin: Supervillin Antibody [NBP1-90363] - Staining of human prostate shows moderate to strong cytoplasmic positivity in smooth muscle cells")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")