EPLIN Antibodies
We offer EPLIN Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each EPLIN Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EPLIN Antibodies can be used in a variety of model species: Human. Use the list below to choose the EPLIN Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EPLIN polyclonal antibodies and browse our EPLIN monoclonal antibody catalog.
Alternate Names for EPLIN Antibodies
anti-EPLIN antibody, anti-LIMA1 antibody, anti-1110021C24Rik antibody, anti-Epithelial protein lost in neoplasm antibody, anti-EPLINepithelial protein lost in neoplasm beta antibody, anti-FLJ38853 antibody, anti-LIM domain and actin binding 1 antibody, anti-MGC131726 antibody, anti-SREBP3LIM domain and actin-binding protein 1 antibody, anti-sterol regulatory element binding protein 3 antibody
12 Results for "eplin" in Primary Antibodies
![Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NB100-2305-img0005.jpg "Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a') In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b',b''). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b'', asterisk). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/9/3/578), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2305-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2305 (lot NB100-2305-4) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by rabbit anti-EPLIN antibody NB100-320. For blotting immunoprecipitated EPLIN, NB100-2305 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunoprecipitation-NB100-2305-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2305_rabbit-polyclonal-eplin-antibody-31020241683934.jpg)
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NB100-2305] - Effects of EGF on EPLIN in IAR-20 cells. (a), (a’) In control IAR-20 cells, EPLIN colocalizes with the circumferential actin bundles at cell-cell boundaries. (b) Addition of EGF leads to release of EPLIN from the zones of disorganization or disappearance of the circumferential bundles (Figure 8b’,b’’). EPLIN colocalizes with the remaining intact circumferential bundle (Figure 8b’’, asterisk). (c) EPLIN fluorescence intensity at the cell-cell boundaries in control & EGF-treated cells. Circles & squares represent individual cells, N = 35, * p < 0.001. (d) Western blot analysis of EPLIN phosphorylation (5% PAAG). Arrows indicate up-shifted bands of phosphorylated EPLIN in the cells treated with EGF. beta -actin was used as loading control. Densitometry results are averaged across three independent experiments. Data are presented as mean ± SEM. (e) MEK inhibitor CI-1040 (4 µM), which inhibits phosphorylation of ERK (p-ERK), significantly decreases the levels of phosphorylated EPLIN at 10 min & 15 min after the addition of EGF. alpha -tubulin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32121325), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2305_rabbit-polyclonal-eplin-antibody-31020241552527.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] -Analysis in human rectum and liver tissues using NBP1-87947 antibody. Corresponding LIMA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-87947_rabbit-polyclonal-eplin-antibody-immunohistochemistry-paraffin-35202475440..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] -Analysis in human rectum and liver tissues using NBP1-87947 antibody. Corresponding LIMA1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human liver, pancreas, rectum and testis using Anti-LIMA1 antibody NBP1-87947 (A) shows similar protein distribution across tissues to independent antibody NBP2-38673 (B).](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP1-87947-img0017.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: EPLIN Antibody [NBP1-87947] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP1-87947-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: EPLIN Antibody [NBP1-87947] - Analysis using Anti-LIMA1 antibody NBP1-87947 (A) shows similar pattern to independent antibody NBP2-38673 (B).](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP1-87947-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP1-87947] - Staining of human cell line U-2 OS shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87947-img0016.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP1-87947-img0013.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human liver.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP1-87947-img0020.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human skin shows moderate membranous and cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87947_rabbit-polyclonal-eplin-antibody-immunohistochemistry-paraffin-35202475610..jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP1-87947] - Staining of human prostate shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-87947_rabbit-polyclonal-eplin-antibody-immunohistochemistry-paraffin-35202475728..jpg)
![Western Blot: EPLIN Antibody [NBP2-38673] - Analysis using Anti-LIMA1 antibody NBP2-38673 (A) shows similar pattern to independent antibody NBP1-87947 (B).](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP2-38673-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: EPLIN Antibody [NBP2-38673] - Analysis using Anti-LIMA1 antibody NBP2-38673 (A) shows similar pattern to independent antibody NBP1-87947 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP2-38673] - Staining of human cell line U-2 OS shows localization to plasma membrane, actin filaments and focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38673-img0009.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human rectum using Anti-LIMA1 antibody NBP2-38673.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0016.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: EPLIN Antibody [NBP2-38673] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP2-38673-img0010.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0006.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human rectum shows high expression.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining in human rectum and pancreas tissues using anti-LIMA1 antibody. Corresponding LIMA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human liver, pancreas, rectum and testis using Anti-LIMA1 antibody NBP2-38673 (A) shows similar protein distribution across tissues to independent antibody NBP1-87947 (B).](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0011.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human testis.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0013.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human liver.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0014.jpg)
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-38673] - Staining of human pancreas using Anti-LIMA1 antibody NBP2-38673.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-38673-img0015.jpg)
![Western Blot: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPLIN.](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Western-Blot-NBP2-45652-img0010.jpg "Western Blot: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPLIN.")
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0009.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0001.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0002.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0003.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0004.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0005.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0006.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0007.jpg)
![Immunohistochemistry: EPLIN Antibody (1A3) [NBP2-45652] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/EPLIN-Antibody-1A3-Immunohistochemistry-NBP2-45652-img0008.jpg)
![Western Blot: EPLIN Antibody (1B7) [H00051474-M06] - Western Blot detection against Immunogen (91.89 KDa) .](http://images.novusbio.com/fullsize/EPLIN-Antibody-1B7-Western-Blot-H00051474-M06-img0001.jpg "Western Blot: EPLIN Antibody (1B7) [H00051474-M06] - Western Blot detection against Immunogen (91.89 KDa) .")
![Immunohistochemistry-Paraffin: EPLIN Antibody (1B7) [H00051474-M06] - Analysis of monoclonal antibody to LIMA1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/EPLIN-Antibody-1B7-Immunohistochemistry-Paraffin-H00051474-M06-img0004.jpg)
![Sandwich ELISA: EPLIN Antibody (1B7) [H00051474-M06] - Detection limit for recombinant GST tagged LIMA1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/EPLIN-Antibody-1B7-Sandwich-ELISA-H00051474-M06-img0003.jpg)
![Western Blot: EPLIN Antibody [NBP3-27896] - Western blot analysis of EPLIN in human colon tissue lysate with EPLIN antibody at (A) 0.5 and (B) 1 u/ml.](http://images.novusbio.com/fullsize/nbp3-27896_rabbit-eplin-pab-2711202419432233.gif "Western Blot: EPLIN Antibody [NBP3-27896] - Western blot analysis of EPLIN in human colon tissue lysate with EPLIN antibody at (A) 0.5 and (B) 1 u/ml.")
![Immunocytochemistry/ Immunofluorescence: EPLIN Antibody [NBP3-27896] - Immunofluorescence of EPLIN in human colon tissue with EPLIN antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp3-27896_rabbit-eplin-pab-2711202420115585.jpg)
![Immunohistochemistry: EPLIN Antibody [NBP3-27896] - Immunohistochemistry of EPLIN in human colon tissue with EPLIN antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp3-27896_rabbit-eplin-pab-2711202419475858.jpg)
![Immunohistochemistry: EPLIN Antibody (1D0) [NBP3-42966] - Immunohistochemistry of paraffin-embedded human heart using (EPLIN antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-42966_mouse-eplin-mab-1d0-101220241715445.jpg "Immunohistochemistry: EPLIN Antibody (1D0) [NBP3-42966] - Immunohistochemistry of paraffin-embedded human heart using (EPLIN antibody) at dilution of 1:50")
![Western Blot: EPLIN Antibody (1D0) [NBP3-42966] - COLO 320 cells were subjected to SDS PAGE followed by western blot with (EPLIN antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-42966_mouse-eplin-mab-1d0-10122024173411.jpg)
![Western Blot: EPLIN Antibody [NBP2-16355] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP2-16355-img0001.jpg "Western Blot: EPLIN Antibody [NBP2-16355] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: EPLIN Antibody [NBP2-16355] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunohistochemistry-Paraffin-NBP2-16355-img0002.jpg)
![Western Blot: EPLIN Antibody [NBP2-16354] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Western-Blot-NBP2-16354-img0001.jpg "Western Blot: EPLIN Antibody [NBP2-16354] - Sample (30 ug of whole cell lysate) A: HeLa B: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: EPLIN Antibody [NBP2-16354] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/EPLIN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16354-img0002.jpg)
![Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2310 (lot NB100-2310-2) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by a previous lot of this antibody (lot NB100-2310-1) and rabbit anti-EPLIN antibody NB100-2305. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EPLIN-Antibody-NB100-2310-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2310 (lot NB100-2310-2) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by a previous lot of this antibody (lot NB100-2310-1) and rabbit anti-EPLIN antibody NB100-2305. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: EPLIN Antibody [NBP3-35184] - Western blot analysis of lysates from A-549 cells, using EPLIN Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35184_rabbit-eplin-pab-912202415161855.jpg "Western Blot: EPLIN Antibody [NBP3-35184] - Western blot analysis of lysates from A-549 cells, using EPLIN Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")