![Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Whole cell lysate (10 ug) from HEK293T, HeLa, NK-92, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/EOMES-Antibody-BLR104H-Western-Blot-NBP3-07014-img0008.jpg "Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Whole cell lysate (10 ug) from HEK293T, HeLa, NK-92, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | BLR104H |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | This product is unpurified. The exact concentration of antibody is not quantifiable. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Immunogen was a peptide representing a region between residue 125 and 175 of human eomesodermin homolog (EOMES) using the numbering given in entry NP_001265111.1 (Gene ID 8320). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EOMES |
| Purity | Tissue culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Storage | Store at 4C. |
| Buffer | Borate Buffered Saline, pH 8.2 |
| Preservative | 0.09% Sodium Azide |
| Concentration | This product is unpurified. The exact concentration of antibody is not quantifiable. |
| Purity | Tissue culture supernatant |
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Secondary Antibodies |
Isotype Controls |
Research Areas for EOMES Antibody (NBP3-07014)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EOMES |
![Immunocytochemistry/Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in NK-92 cells by ICC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/EOMES-Antibody-BLR104H-Immunocytochemistry-Immunofluorescence-NBP3-07014-img0007.jpg "Immunocytochemistry/Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in NK-92 cells by ICC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in tonsil by IHC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/EOMES-Antibody-BLR104H-Immunohistochemistry-NBP3-07014-img0006.jpg "Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in tonsil by IHC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.")
![Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Detection of human EOMES by WB of immunoprecipitates from NK-92 cell lysate. Antibodies: NBP3-07014 and rabbit anti-EOMES BL24367.](http://images.novusbio.com/fullsize/EOMES-Antibody-BLR104H-Western-Blot-NBP3-07014-img0005.jpg "Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Detection of human EOMES by WB of immunoprecipitates from NK-92 cell lysate. Antibodies: NBP3-07014 and rabbit anti-EOMES BL24367.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")