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EOMES Antibodies

We offer EOMES Antibodies for use in common research applications: ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Intracellular Staining by Flow Cytometry, Sandwich ELISA, Western Blot. Each EOMES Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our EOMES Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the EOMES Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our EOMES polyclonal antibodies and browse our EOMES monoclonal antibody catalog.

Alternate Names for EOMES Antibodies

anti-EOMES antibody, anti-eomesodermin (Xenopus laevis) homolog antibody, anti-eomesodermin antibody, anti-t box, brain, 2 antibody, anti-T-box brain protein 2 antibody, anti-T-box brain2 antibody, anti-T-brain-2 antibody, anti-TBR-2 antibody, anti-TBR2eomesodermin homolog antibody

9 Results for "eomes" in Primary Antibodies

AF6166

EOMES Antibody

(26 Publications)

100 μg

AF6166

Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesoendoderm differentiated (+). PVDF Membrane was probed with 1 µg/mL of Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for EOMES at approximately 85-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

BG01V human embryonic stem cells differentiated to mesendoderm were stained with Sheep Anti-Human EOMES Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6166, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6166-SP

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MAB6166

EOMES Antibody (644730)

(2 Reviews)

(12 Publications)

100 μg

MAB6166

Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesendoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for EOMES at approximately 100 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Monoclonal Antibody (Catalog # MAB6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

BG01V human embryonic stem cells differentiated to mesendoderm were stained with Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC6166U-100UG
Alexa Fluor 405	IC6166V-100UG
Alexa Fluor 488	IC6166G
Alexa Fluor 532	FAB6166X
Alexa Fluor 594	IC6166T-100UG
Alexa Fluor 647	IC6166R-100UG
Alexa Fluor 700	IC6166N-100UG
Alexa Fluor 750	IC6166S-100UG
Allophycocyanin	IC6166A
Biotin	FAB6166B
CoraFluor 1	FAB6166CL1
DyLight 350	FAB6166D
DyLight 405	FAB6166E
DyLight 488	FAB6166K
DyLight 550	FAB6166L
DyLight 594	FAB6166M
DyLight 650	FAB6166W
DyLight 680	FAB6166Y
DyLight 755	FAB6166Z
FITC	FAB6166F
HRP	FAB6166H
Janelia Fluor 525	FAB6166JF525
Janelia Fluor 549	FAB6166I
Janelia Fluor 646	FAB6166J
Janelia Fluor 669	FAB6166JF669
PE	FAB6166P
PerCP	FAB6166C
mFluor Violet 450 SE	FAB6166MFV450
mFluor Violet 500 SE	FAB6166MFV500
mFluor Violet 610 SE	FAB6166MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC6166A
Carrier Free	IC6166G
Carrier Free	MAB6166-SP
Carrier Free	IC6166V-100UG
Carrier Free	IC6166U-100UG
Carrier Free	IC6166T-100UG
Carrier Free	IC6166S-100UG
Carrier Free	IC6166R-100UG
Carrier Free	IC6166N-100UG

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NBP1-80699

EOMES Antibody

(1 Review)

(4 Publications)

0.1 ml

NBP1-80699

Immunohistochemistry-Paraffin: EOMES Antibody [NBP1-80699] - Staining of human lymph node tissues shows moderate nuclear positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: EOMES Antibody [NBP1-80699] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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MAB8889

EOMES Antibody (1219A) [Unconjugated]

(5 Publications)

100 μg

MAB8889

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Monoclonal Antibody (Catalog # MAB8889) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC8889U-100UG
Alexa Fluor 405	IC8889V-100UG
Alexa Fluor 488	IC8889G-100UG
Alexa Fluor 532	FAB8889X
Alexa Fluor 594	IC8889T-100UG
Alexa Fluor 647	IC8889R-100UG
Alexa Fluor 700	IC8889N-100UG
Alexa Fluor 750	IC8889S-100UG
Allophycocyanin	FAB8889A
Allophycocyanin/Cy7	FAB8889APCCY7
Biotin	FAB8889B
CoraFluor 1	FAB8889CL1
DyLight 350	FAB8889D
DyLight 405	FAB8889E
DyLight 488	FAB8889K
DyLight 550	FAB8889L
DyLight 594	FAB8889M
DyLight 650	FAB8889W
DyLight 680	FAB8889Y
DyLight 755	FAB8889Z
FITC	FAB8889F
HRP	FAB8889H
Janelia Fluor 525	FAB8889JF525
Janelia Fluor 549	FAB8889I
Janelia Fluor 646	FAB8889J
Janelia Fluor 669	FAB8889JF669
PE/Atto594	FAB8889PEATT594
PE/Cy5.5	FAB8889PECY55
PE/Cy7	FAB8889PECY7
PerCP	FAB8889C
mFluor Violet 450 SE	FAB8889MFV450
mFluor Violet 500 SE	FAB8889MFV500
mFluor Violet 610 SE	FAB8889MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC8889G-100UG
Carrier Free	MAB8889-SP
Carrier Free	IC8889V-100UG
Carrier Free	IC8889U-100UG
Carrier Free	IC8889T-100UG
Carrier Free	IC8889S-100UG
Carrier Free	IC8889R-100UG
Carrier Free	IC8889N-100UG

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NBP3-43668

EOMES Antibody (CL15426) [Unconjugated]

100 ul

NBP3-43668

Staining of rat cerebellum shows strong nuclear positivity in cells in the granular layer.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of mouse embryo, E14 shows strong nuclear positivity in the developing cerebral cortex.

Staining of human kidney shows no positivity in cells in tubules as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human tonsil and kidney tissues using NBP3-43668 antibody. Corresponding EOMES RNA-seq data are presented for the same tissues.

Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.

Staining of human cerebellum shows moderate to strong nuclear positivity in a subset of cells in granular layer.

Reactivity:

Hu, Mu, Rt, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt, Mu, Rt

Applications:

Applications:

Host:

MOUSE Monoclonal

Host: MOUSE Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-07014

EOMES Antibody (BLR104H)

100 μg

NBP3-07014

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Whole cell lysate (10 ug) from HEK293T, HeLa, NK-92, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

Immunocytochemistry/Immunofluorescence: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in NK-92 cells by ICC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry: EOMES Antibody (BLR104H) [NBP3-07014] - Human EOMES in tonsil by IHC. Antibody: Rabbit anti-EOMES recombinant monoclonal antibody [BLR104H]. Secondary: HRP-conjugatedgoat anti-rabbit IgG. Substrate: DAB.

Western Blot: EOMES Antibody (BLR104H) [NBP3-07014] - Detection of human EOMES by WB of immunoprecipitates from NK-92 cell lysate. Antibodies: NBP3-07014 and rabbit anti-EOMES BL24367.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-43667

EOMES Antibody (CL15425) [Unconjugated]

100 ul

NBP3-43667

Staining of human prostate shows no positivity in glandular cells as expected.

<b>Orthogonal Strategies Validation. </b>Analysis in human tonsil and prostate tissues using NBP3-43667 antibody. Corresponding EOMES RNA-seq data are presented for the same tissues.

Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.

Staining of mouse brain shows moderate to strong nuclear positivity in a subset of neurons in the cerebellum.

Staining of human cerebellum shows strong nuclear positivity in a subset of neurons in the granular layer.

Staining of mouse embryo, E14 shows moderate to strong nuclear positivity in neuronal cells in the developing cortex.

Reactivity:

Hu, Mu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Mu, Rt

Applications:

Applications:

Host:

MOUSE Monoclonal

Host: MOUSE Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-13356

EOMES Antibody

100 ul

NBP3-13356

Western Blot: EOMES Antibody [NBP3-13356] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EOMES antibody (NBP3-13356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: EOMES Antibody [NBP3-13356] - EOMES antibody detects EOMES protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EOMES stained by EOMES antibody (NBP3-13356) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: EOMES Antibody [NBP3-13356] - EOMES antibody detects TBR2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. TBR2 stained by EOMES antibody (NBP3-13356) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: EOMES Antibody [NBP3-13356] - EOMES antibody detects TBR2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. TBR2 stained by EOMES antibody (NBP3-13356) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-13136

EOMES Antibody

100 ul

NBP3-13136

Western Blot: EOMES Antibody [NBP3-13136] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EOMES antibody (NBP3-13136) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: EOMES Antibody [NBP3-13136] - EOMES antibody detects EOMES protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. EOMES stained by EOMES antibody (NBP3-13136) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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