![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0009.jpg "Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IP |
| Clone | 30A882.1.1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A full-length human Bif-1 recombinant protein was used as immunogen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SH3GLB1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Immunohistochemistry-Paraffin-NBP2-24733-img0008.jpg "Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz.")
![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0006.jpg "Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.")
![Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Simple-Western-NBP2-24733-img0004.jpg "Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
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