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![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0009.jpg "Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Amino acid E28 on Bif-1 is important for calpain-mediated processing and autophagic clearance of p62. The myc-tagged constructs: wild-type Endophilin B1/Bif-1 and E28A Endophilin B1/Bif-1 were transfected into H1299 cells. 24h later, the cells were treated or not with 100nM thapsigargin for 1h. Afterwards, the cell lysates were prepared and analyzed by western blot with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//bio.biologists.org/content/6/5/551.long), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 5 ug/ml conc. of Endophilin B1 antibody (clone 30A882.1.1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Endophilin B1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Immunohistochemistry-Paraffin-NBP2-24733-img0008.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Western-Blot-NBP2-24733-img0006.jpg)
![Simple Western: Endophilin B1/Bif-1 Antibody (30A882.1.1) [NBP2-24733] - Simple Western lane view shows a specific band for Endophilin B1/Bif-1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-30A882-1-1-Simple-Western-NBP2-24733-img0004.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of NIH3T3 (A), RAW264.7 (B) and KNRK (C) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NB100-820-img0002.jpg "Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of NIH3T3 (A), RAW264.7 (B) and KNRK (C) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of paraffin embedded Human Colon. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NB100-820-img0004.jpg)
![Flow Cytometry: Endophilin B1/Bif-1 Antibody [NB100-820] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Flow-Cytometry-NB100-820-img0003.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NB100-820] - Staining of HEK293 (A) and A431 (B) cell lysate with antibody at 0.1 ug/mL. RIPA buffer, 35 ug total protein per lane. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NB100-820-img0001.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NBP1-31130-img0012.jpg "Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Endophilin B1/Bif-1 antibody [C2C3], C-term detects Bif-1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.Green: Bif-1 protein stained by Bif-1 antibody [C2C3], C-term (NBP1-31130).Blue: Hoechst 33342 staining.Scale bar = 10 um](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31130-img0013.jpg)
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Bif-1 antibody detects Bif-1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bif-1 antibody (NBP1-31130).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-31130-img0014.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-31130] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NBP1-31130-img0011.jpg)
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Staining in human testis and pancreas tissues using anti-SH3GLB1 antibody. Corresponding SH3GLB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89972-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Staining in human testis and pancreas tissues using anti-SH3GLB1 antibody. Corresponding SH3GLB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NBP1-89972-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89972-img0011.jpg)
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89972-img0007.jpg)
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89972] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89972-img0008.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NBP1-89973-img0005.jpg "Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89973-img0006.jpg)
![Western Blot: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-62](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Western-Blot-NBP1-89973-img0004.jpg)
![Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Staining of human testis shows distinct cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89973-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endophilin B1/Bif-1 Antibody [NBP1-89973] - Staining in human testis and pancreas tissues using anti-SH3GLB1 antibody. Corresponding SH3GLB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endophilin-B1-Bif-1-Antibody-Immunohistochemistry-Paraffin-NBP1-89973-img0007.jpg)
![Western Blot: Bi-f-1/Endophilin-B1 Overexpression Lysate (Adult Normal) [NBL1-15933] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bi-f-1/Endophilin-B1.](http://images.novusbio.com/fullsize/Bi-f-1-Endophilin-B1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15933-img0002.jpg "Western Blot: Bi-f-1/Endophilin-B1 Overexpression Lysate (Adult Normal) [NBL1-15933] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bi-f-1/Endophilin-B1.")
![SDS-Page: Bi-f-1/Endophilin-B1 Protein [NBP2-23467]](http://images.novusbio.com/fullsize/Bi-f-1-Endophilin-B1-Protein-SDS-Page-NBP2-23467-img0001.jpg "SDS-Page: Bi-f-1/Endophilin-B1 Protein [NBP2-23467]")