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eIF4EBP2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: eIF4EBP2 Antibody [NBP2-37985] - Staining of human cell line A-431 shows localization to nucleoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: eIF4EBP2 Antibody [NBP2-37985] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: eIF4EBP2 Antibody [NBP2-37985] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: eIF4EBP2 Antibody [NBP2-37985] - Staining of human lower gastrointestinal shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: eIF4EBP2 Antibody [NBP2-37985] - Staining of human endometrium shows moderate to strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: eIF4EBP2 Antibody [NBP2-37985] - Staining of human upper gastrointestinal shows moderate to strong granular cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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eIF4EBP2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HQPSQSRAIPTRTVAISDAAQLPHDYCTTP
Predicted Species
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4EBP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
eIF4EBP2 Protein (NBP2-37985PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for eIF4EBP2 Antibody

  • 4EBP2
  • 4E-BP2
  • eIF4E-binding protein 2
  • eukaryotic translation initiation factor 4E binding protein 2
  • eukaryotic translation initiation factor 4E-binding protein 2
  • PHASII
  • phosphorylated, heat and acid stable regulated by insulin protein II

Background

EIF4EBP2 regulates eIF4E activity by preventing its assembly into the EIF4F complex. It mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. Nonphosphorylated EIF4EBP2 interacts with EIF4E.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF4EBP2
Uniprot