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MNK1 Antibody (2F12)

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Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Old wild type and p38 alpha knock out livers were Western blotted for MNK1 and phosphorylated MNK1 on Thr197/202. Alpha tubulin was used as a loading control. Densitometric quantification of p-MNK-1(T197/202)/MNK1 were determined. Data are shown as mean +/- SD. **P < 0.01 WT versus KO. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0171738) licensed under a CC-BY license.
Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Old wild type and p38 alpha knock out livers were Western blotted for MNK1 and phosphorylated MNK1 on Thr197/202. Alpha tubulin was used as a loading control. ...read more
Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml
Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - detection against Immunogen (77.26 KDa) .
Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of MKNK1 expression in Raw 264.7. (Isoform: 51.342, 47.402, 39.103 kD)
p38alpha-mediated phosphorylation pathways. b) Old wild type and p38alpha knock out livers were Western blotted for MNK1 and phosphorylated MNK1 on Thr197/202, for MK2 and phosphorylated MK2 on Thr334 and Thr222, for ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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MNK1 Antibody (2F12) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
Specificity
MAP kinase interacting serine/threonine kinase 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MKNK1
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publication using
H00008569-M14 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MNK1 Antibody (2F12)

  • EC 2.7.11
  • EC 2.7.11.1
  • MAP kinase interacting kinase 1
  • MAP kinase interacting serine/threonine kinase 1
  • MAP kinase signal-integrating kinase 1
  • MAP kinase-interacting serine/threonine-protein kinase 1
  • MKNK1
  • MNK1
  • MNK1MAP kinase-interacting serine/threonine kinase 1

Background

MNK1 may play a role in the response to environmental stress and cytokines. It appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MNK1 Antibody
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H00008569-M14
MNK1 Antibody (2F12)
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC

Publications for MNK1 Antibody (H00008569-M14)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol MKNK1
Entrez
OMIM
Uniprot