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eIF4B Antibody (1F5) - BSA Free

Name: eIF4B Antibody (1F5) - BSA Free
Brand: Novus Biologicals
SKU: NBP2-37488
Price: 449 USD

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Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.

Immunocytochemistry/ Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more

Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.

Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).

Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)

ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary

Reactivity	Hu, MuSpecies Glossary
Applications	WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone	1F5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated
Format	BSA Free
Concentration	1 mg/ml

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Catalog# & Formulation	Size	Price
BSA Free NBP2-37488-0.025ml

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eIF4B Antibody (1F5) - BSA Free Summary

Immunogen	Purified recombinant fragment of human eIF4B expressed in E. Coli.
Isotype	IgG2b
Clonality	Monoclonal
Host	Mouse
Gene	EIF4B
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	CyTOF-ready ELISA 1:10000 Flow Cytometry 1:200 - 1:400 Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000 Immunohistochemistry 1:200 - 1:1000 Immunohistochemistry-Paraffin 1:200 - 1:1000 Western Blot 1:500 - 1:2000
Application Notes	This antibody is Cytof ready.
Theoretical MW	80 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS
Preservative	0.05% Sodium Azide
Concentration	1 mg/ml
Purity	Protein G purified

Alternate Names for eIF4B Antibody (1F5) - BSA Free

Eif4a2
eIF4B
EIF-4B
eukaryotic translation initiation factor 4B
PRO1843

Background

Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

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Bioinformatics

Gene Symbol

EIF4B

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

eIF4B Antibody (1F5) - BSA Free

Species Glossary

Images

Product Details

Order Details

eIF4B Antibody (1F5) - BSA Free Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Alternate Names for eIF4B Antibody (1F5) - BSA Free

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for eIF4B Antibody (NBP2-37488) (0)

Reviews for eIF4B Antibody (NBP2-37488) (0)

Product General Protocols

Video Protocols

FAQs for eIF4B Antibody (NBP2-37488) (0)

Secondary Antibodies

Isotype Controls

Additional eIF4B Products

Research Areas for eIF4B Antibody (NBP2-37488)

Blogs on eIF4B

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics