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![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0003.jpg "Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mouse mAb against A549 (1), A431 (2), HepG2 (3), HEK293 (4), HeLa (5), Jurkat (6), K562 (7), NIH3T3 (8), and MCF-7 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody (1F5) [NBP2-37488] - Immunofluorescence analysis of NIH3T3 cells using EIF4B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunocytochemistry-Immunofluorescence-NBP2-37488-img0004.jpg)
![Immunohistochemistry: eIF4B Antibody (1F5) [NBP2-37488] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using EIF4B mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Immunohistochemistry-NBP2-37488-img0006.jpg)
![Flow Cytometry: eIF4B Antibody (1F5) [NBP2-37488] - Flow cytometric analysis of HeLa cells using EIF4B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Flow-Cytometry-NBP2-37488-img0005.jpg)
![Western Blot: eIF4B Antibody (1F5) [NBP2-37488] - Western blot analysis using EIF4B mAb against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-Western-Blot-NBP2-37488-img0002.jpg)
![ELISA: eIF4B Antibody (1F5) [NBP2-37488] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF4B-Antibody-1F5-ELISA-NBP2-37488-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-16306] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16306-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306] - Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-16306-img0006.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0001.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0003.jpg)
![Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-16306-img0004.jpg)
![Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NB100-93308-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.")
![Western Blot: eIF4B Antibody [NBP2-24475] - analysis of eIF4B in Hela cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody. 25 ug/ml.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-24475-img0001.jpg "Western Blot: eIF4B Antibody [NBP2-24475] - analysis of eIF4B in Hela cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody. 25 ug/ml.")
![Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-288202412461610.jpg "Western Blot: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Western blot analysis of eIF4B on different lysates with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/50,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T treated with 100nM Calyculin A for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 100ng/mL Calyculin A for 30 minutes cell lysate Lane 5: Jurkat cell lysate Lane 6: Jurkat treated with 100nM Calyculin A for 30 minutes cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 9: PC-12 cell lysate Lane 10: PC-12 treated with 100nM Calyculin A for 30 minutes cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 69 kDa Observed band size: 80 kDa Exposure time: 21 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32763) at 1/50,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32763) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29820241411549.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling eIF4B with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF4B antibody (NBP3-32763) at 1/20,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202410484885.jpg)
![Flow Cytometry: eIF4B [p Ser406] Antibody (JE63-80) [NBP3-32763] - Flow cytometric analysis of Jurkat cells labeling eIF4B. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32763, 1/5,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32763_rabbit-eif4b-p-ser406-mab-je63-80-29202416453295.jpg)
![Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-24504-img0001.jpg "Western Blot: eIF4B Antibody [NBP2-24504] - analysis of eIF4B in K562 cell lysate in the 1) absence and2) presence of immunizing peptide, and 3) RAW cell lysate using this antibody. 005 ug/ml, 0.005 ug/ml and 1.0 ug/ml respectively.")
![Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/eIF4B-Antibody-Western-Blot-NBP2-33284-img0005.jpg "Western Blot: eIF4B Antibody [NBP2-33284] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-33284] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33284-img0008.jpg)
![Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-33284] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF4B-Antibody-Immunohistochemistry-Paraffin-NBP2-33284-img0007.jpg)
![Recombinant Human eIF4B Protein [H00001975-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001975-P01-1.jpg "Recombinant Human eIF4B Protein [H00001975-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: eIF4B Antibody (S07-2H2) [NBP3-19859] - Western blot detection of eIF4B in Hela, A549, HL-60, U2OS cell lysates using NBP3-19859 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/eIF4B-Antibody-S07-2H2-Western-Blot-NBP3-19859-img0002.jpg "Western Blot: eIF4B Antibody (S07-2H2) [NBP3-19859] - Western blot detection of eIF4B in Hela, A549, HL-60, U2OS cell lysates using NBP3-19859 (1:1000 diluted). Predicted band size: 69kDa. Observed band size: 80kDa.")
![Immunohistochemistry-Paraffin: eIF4B Antibody (S07-2H2) [NBP3-19859] - Immunohistochemistry of eIF4B in paraffin-embedded Human lung cancer tissue using NBP3-19859 at dilution 1/50](http://images.novusbio.com/fullsize/eIF4B-Antibody-S07-2H2-Immunohistochemistry-Paraffin-NBP3-19859-img0001.jpg)
![Western Blot: eIF4B [p Ser 406] Antibody (SR2202) [NBP3-21605] - Western blot analysis using NBP3-21605 in (1) HeLa cell lysate; (2) HeLa cell treated with Alkaline Phosphatase lysate.](http://images.novusbio.com/fullsize/nbp3-21605_rabbit-eif4b-p-ser-406-mab-sr2202-57202317215166.jpg "Western Blot: eIF4B [p Ser 406] Antibody (SR2202) [NBP3-21605] - Western blot analysis using NBP3-21605 in (1) HeLa cell lysate; (2) HeLa cell treated with Alkaline Phosphatase lysate.")