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eEF-2 Antibody (4E2Z5)

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Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of various cell lines, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of U-2 OS cells using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunohistochemistry of paraffin-embedded human appendix using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Western Blot: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Western blot analysis of extracts of Rat spleen, using eEF-2 Rabbit mAb (NBP3-16866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of C6 cells using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: eEF-2 Antibody (4E2Z5) [NBP3-16866] - Immunofluorescence analysis of NIH-3T3 cells using eEF-2 Rabbit mAb (NBP3-16866) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
4E2Z5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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eEF-2 Antibody (4E2Z5) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 759-858 of human eEF-2 (P13639). IYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EEF2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for eEF-2 Antibody (4E2Z5)

  • eEF2
  • eEF-2
  • EF-2
  • EF2EEF-2
  • elongation factor 2
  • eukaryotic translation elongation factor 2
  • polypeptidyl-tRNA translocase

Background

The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eEF-2 Antibody (NBP3-16866) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EEF2