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EGLN3/PHD3 Antibody [mFluor Violet 450 SE]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IP, MS, KD, MS
Clonality
Polyclonal
Host
Rabbit
Conjugate
mFluor Violet 450 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-139AF350
Alexa Fluor 405
NB100-139AF405
Alexa Fluor 488
NB100-139AF488
Alexa Fluor 532
NB100-139AF532
Alexa Fluor 594
NB100-139AF594
Alexa Fluor 647
NB100-139AF647
Alexa Fluor 700
NB100-139AF700
Alexa Fluor 750
NB100-139AF750
Allophycocyanin
NB100-139APC
Biotin
NB100-139B
CoraFluor 1
NB100-139CL1
DyLight 350
NB100-139UV
DyLight 405
NB100-139V
DyLight 488
NB100-139G
DyLight 550
NB100-139R
DyLight 594
NB100-139DL594
DyLight 650
NB100-139C
DyLight 680
NB100-139FR
DyLight 755
NB100-139IR
FITC
NB100-139F
HRP
NB100-139H
Janelia Fluor 525
NB100-139JF525
Janelia Fluor 549
NB100-139JF549
Janelia Fluor 646
NB100-139JF646
Janelia Fluor 669
NB100-139JF669
PE
NB100-139PE
PerCP
NB100-139PCP
Unconjugated
NB100-139
mFluor Violet 500 SE
NB100-139MFV500
mFluor Violet 610 SE
NB100-139MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-139
BSA Free
NB100-139SS
BSA Free
NB100-139
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EGLN3/PHD3 Antibody [mFluor Violet 450 SE] Summary

Immunogen
Synthetic peptide corresponding to residues between 50-100 of human PHD3/HIF Prolyl Hydroxylase 3 using the numbering given in entry NP_071356.1 (GeneID 112399).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Mass Spectrometry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24037093)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for EGLN3/PHD3 Antibody [mFluor Violet 450 SE]

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EGLN3/PHD3 Antibody [mFluor Violet 450 SE]
Species: Hu, Mu, Rt
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Bioinformatics

Gene Symbol EGLN3