EGF Antibody [Biotin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha , amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin-binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ELISA Development, Flow Cytometry (CAR-T).

Showing Publications 1 - 10 of 10.

Publications using BAF236	Applications	Species
Sophie Papa, Antonella Adami, Michael Metoudi, Richard Beatson, Molly Sarah George, Daniela Achkova et al. Intratumoral pan-ErbB targeted CAR-T for head and neck squamous cell carcinoma: interim analysis of the T4 immunotherapy study Journal for ImmunoTherapy of Cancer 6/15/2023 [PMID: 37321663]
Daniel Larcombe-Young, Lynsey Whilding, David Marc Davies, Benjamin Draper, Natasha Bechman, John Maher Generation of human parallel chimeric antigen receptor (pCAR) T cells to achieve synergistic T cell co-stimulation STAR Protocols 6/1/2022 [PMID: 35620078]
Ana C. Parente-Pereira, Richard E. Beatson, David M. Davies, Caroline Hull, Lynsey M. Whilding, Joanna C. Porter et al. Generation and application of TGF beta -educated human V gamma 9Vδ2 T cells STAR Protocols 6/1/2022 [PMID: 35496793]
Tamara Muliaditan, Leena Halim, Lynsey M. Whilding, Benjamin Draper, Daniela Y. Achkova, Fahima Kausar et al. Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors Cell Reports Medicine 12/21/2021 [PMID: 35028604]
Paris Kosti, Karen I. Larios-Martinez, John Maher, James N. Arnold Generation of hypoxia-sensing chimeric antigen receptor T cells STAR Protocols 9/1/2021 [PMID: 34401788]
MCI van Schalk, SJC van der St, L Bosshard-C, H Graves, S Papa, AC Parente-Pe, F Farzaneh, CD Fisher, A Hope, A Adami, J Maher Development and Validation of a Good Manufacturing Process for IL-4-Driven Expansion of Chimeric Cytokine Receptor-Expressing CAR T-Cells Cells, 2021-07-15;10(7):. 2021-07-15 [PMID: 34359966] (Flow Cytometry (CAR-T), Human)	Flow Cytometry (CAR-T)	Human
Kosti P, Opzoomer J, Larios-Martinez K et al. Hypoxia-sensing CAR T cells provide safety and efficacy in treating solid tumors Cell Reports Medicine 4/1/2021 [PMID: 33948568]
Sjoukje J. C. van der Stegen, David M. Davies, Scott Wilkie, Julie Foster, Jane K. Sosabowski, Jerome Burnet et al. Preclinical In Vivo Modeling of Cytokine Release Syndrome Induced by ErbB-Retargeted Human T Cells: Identifying a Window of Therapeutic Opportunity? The Journal of Immunology 11/1/2013 [PMID: 24062490]
Frank Götschel, Daniela Berg, Wolfgang Gruber, Christian Bender, Markus Eberl, Myriam Friedel et al. Synergism between Hedgehog-GLI and EGFR Signaling in Hedgehog-Responsive Human Medulloblastoma Cells Induces Downregulation of Canonical Hedgehog-Target Genes and Stabilized Expression of GLI1 PLoS ONE 6/10/2013 [PMID: 23762360]
Wirth S, Segretin ME, Mentaberry A, Bravo-Almonacid F Accumulation of hEGF and hEGF-fusion proteins in chloroplast-transformed tobacco plants is higher in the dark than in the light. J. Biotechnol., 2006-04-03;125(2):159-72. 2006-04-03 [PMID: 16584796] (ELISA Development, Human)	ELISA Development	Human

Array BAF236

• EGF Antibodies
• EGF ELISA Kit
• EGF Lysate
• EGF Proteins
• EGF ELISAs
• EGF Proteins and Enzymes