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Eg5 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Analysis in human tonsil and pancreas tissues. Corresponding KIF11 RNA-seq data are presented for the same tissues.
Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human tonsil shows moderate to strong cytoplasmic positivity, mainly in germinal center cells.
Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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Eg5 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF11
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Eg5 Protein (NBP1-84887PEP)
Publications
Read Publication using NBP1-84887.

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24637494)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Eg5 Antibody

  • Eg5
  • HKSP
  • KIF11
  • kinesin family member 11
  • kinesin-like 1
  • Kinesin-like protein 1
  • kinesin-like protein KIF11
  • Kinesin-like spindle protein HKSP
  • Kinesin-related motor protein Eg5
  • KNSL1
  • KNSL1TRIP-5
  • thyroid receptor interacting protein 5
  • Thyroid receptor-interacting protein 5
  • TR-interacting protein 5
  • TRIP5
  • TRIP-5
  • TRIP5EG5

Background

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eg5 Antibody (NBP1-84887)(1)

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Bioinformatics

Gene Symbol KIF11