Immunohistochemistry-Paraffin: KIFC1 Antibody [NB100-40844] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- KIFC1/MKLP2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: KIFC1 Antibody [NB100-40844] - Detection of Human KIFC1/MKLP2 on HeLa whole cell lysate using NB100-40844. KIFC1/MKLP2 was also immunoprecipitated by other rabbit anti-KIFC1/MKLP2 antibodies and ...read more
The immunogen recognized by this antibody maps to a region between residue 625 and 673 of human Kinesin Family Member C1 (Mitotic Kinesin-Like Protein 2) using the numbering given in entry NP_002254.1 (GeneID 3833).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIFC1
Purity
Immunogen affinity purified
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Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. WB reactivity reported in (PMID: 28430595). Western Blot Not recommended. Chip, WB reactivity reported in (PMID: 24648347). Use in ICC/IF was reported in scientific literature (PMID: 30804089).
Theoretical MW
74 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-40844 in the following applications:
Rat reactivity reported in scientific literature (PMID: 30804089).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for KIFC1 Antibody
HSETMGC1202
kinesin family member C1
kinesin-like 2
Kinesin-like protein 2
kinesin-like protein KIFC1
Kinesin-related protein HSET
KNSL2MGC149736
MGC149737
Background
KIFC1 is a C-terminal kinesin motor associated with the nuclear membrane and acrosome in round and elongating spermatids. This location in developing spermatids is consistent with possible roles in acrosome elongation and manchette motility or both. It is a minus-end-directed motor enzyme.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
PARVIN A, WEI B, HAO S Et al. KIFC1 overexpression promotes prostate cancer cell survival and proliferation in vitro by clustering of amplified centrosomes via interaction with Centrin 2 BIOCELL 2021-07-22 (WB, Human)
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