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EDF1 Antibody - BSA Free

Images

 
Western Blot: EDF1 Antibody [NBP1-84011] - Analysis in human cell line MCF-7.
Immunocytochemistry/ Immunofluorescence: EDF1 Antibody [NBP1-84011] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human lymph node showing strong nuclear and cytoplasmic postitivity in geminal and non-geminal center cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human duodenum shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human skin showing nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Staining of human cerebellum showing strong nuclear and cytoplamic positivity in Purkinje cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

EDF1 Antibody - BSA Free Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIE
Predicted Species
Mouse (99%), Rat (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EDF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
EDF1 Protein (NBP1-84011PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EDF1 Antibody - BSA Free

  • EDF-1Multiprotein-bridging factor 1
  • endothelial differentiation-related factor 1
  • MBF1
  • MGC9058
  • multiprotein bridging factor 1
  • multiprotein bridging factor-1

Background

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EDF1