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PCP4 Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: PCP4 Antibody [NBP1-80929] - Staining in human cerebral cortex and liver tissues . Corresponding PCP4 RNA-seq data are presented for the same tissues.
Immunohistochemistry: PCP4 Antibody [NBP1-80929] - Staining of mouse cerebral cortex shows strong positivity in the deep layers neurons.
Immunohistochemistry-Paraffin: PCP4 Antibody [NBP1-80929] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PCP4 Antibody [NBP1-80929] - Staining of human cerebral cortex shows moderate cytoplasmic positivity.
Immunohistochemistry: PCP4 Antibody [NBP1-80929] - Staining of mouse cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PCP4 Antibody [NBP1-80929] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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PCP4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GAGATNGKDKTSGENDGQKKVQEEFDIDMDAPETERAAVAIQSQFRKFQKKK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PCP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
PCP4 Protein (NBP1-80929PEP)
Publications
Read Publications using
NBP1-80929 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26214372).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PCP4 Antibody

  • brain specific polypeptide PEP19, 10Brain-specific antigen PCP-4
  • Brain-specific polypeptide PEP-19
  • PEP19
  • PEP-19
  • Purkinje cell protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PCP4 Antibody (NBP1-80929)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: IF/IHC.


Filter By Application
IF/IHC
(4)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-80929 Applications Species
Manuel Valero, Elena Cid, Robert G Averkin et al. Determinants of different deep and superficial CA1 pyramidal cell dynamics during sharp-wave ripples. Nature Neuroscience 2015-07-27 [PMID: 26214372] (IF/IHC, Rat) IF/IHC Rat
Zhou J, Shaikh LH, Neogi SG et al. DACH1, a Zona Glomerulosa Selective Gene in the Human Adrenal, Activates Transforming Growth Factor--beta Signaling and Suppresses Aldosterone Secretion. Hypertension 2015-05-01 [PMID: 25776071] (IF/IHC, Human) IF/IHC Human
Botcher NA, Falck JE, Thomson AM, Mercer A. Distribution of interneurons in the CA2 region of the rat hippocampus. Front Neuroanat 2014-01-01 [PMID: 25309345] (IF/IHC, Rat) IF/IHC Rat
Hitti FL, Siegelbaum SA. The hippocampal CA2 region is essential for social memory. Nature 2014-04-03 [PMID: 24572357]
Kohara K, Pignatelli M, Rivest AJ et al. Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits. Nat Neurosci 2014-02-01 [PMID: 24336151]
Souali-Crespo S, Condrea D, Vernet N et al. Loss of NR5A1 in Sertoli cells after sex determination changes their cellular identity and induces their death by anoikis bioRxiv 2023-01-07
Martikainen M, Lugano R, Pietilä I et al. VLDLR mediates alphavirus neuroinvasion through the blood-cerebrospinal fluid barrier Research Square 2023-10-30
Ben-Simon Y, Kaefer K, Velicky P et al. A direct excitatory projection from entorhinal layer 6b neurons to the hippocampus contributes to spatial coding and memory Nature communications 2022-08-16 [PMID: 35974109] (IF/IHC, Mouse)

Details:
Supplementary Figure 5: Dilutions: 1:500
IF/IHC Mouse

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Secondary Antibodies

 

Isotype Controls

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Blogs on PCP4.

RNA-binding protein Staufen1 conspires with Atxn2 in stress granules to cause neurodegeneration by dysregulating RNA metabolism
By Jamshed Arslan Pharm.D. Spinocerebellar ataxia type 2 (SCA2) is a movement disorder characterized by neurodegeneration. The cause of this autosomal dominant disease is a mutation in the RNA processing gene Atxn2,...  Read full blog post.

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Bioinformatics

Gene Symbol PCP4
Entrez
Uniprot