![Western Blot: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Western-Blot-NBP2-16263-img0006.jpg "Western Blot: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody diluted by 1:5000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ECHS1. The exact sequence is proprietary. |
| Localization | Mitochondrion matrix |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ECHS1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ECHS1 Antibody (NBP2-16263)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16263-img0012.jpg "Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0009.jpg "Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.")
![Western Blot: ECHS1 Antibody [NBP2-16263] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Western-Blot-NBP2-16263-img0001.jpg "Western Blot: ECHS1 Antibody [NBP2-16263] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ECHS1 Antibody [NBP2-16263] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Western-Blot-NBP2-16263-img0002.jpg "Western Blot: ECHS1 Antibody [NBP2-16263] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16263-img0004.jpg "Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.")
![Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16263-img0010.jpg "Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0003.jpg "Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using antibody at 1:250 dilution.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0005.jpg "Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0007.jpg "Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0008.jpg "Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 antibody dilution: 1:500.")
![Immunoprecipitation: ECHS1 Antibody [NBP2-16263] - Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 ug of ECHS1 antibody. Western blot analysis was performed using ECHS1 antibody.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunoprecipitation-NBP2-16263-img0011.jpg "Immunoprecipitation: ECHS1 Antibody [NBP2-16263] - Immunoprecipitation of ECHS1 protein from 293T whole cell extracts using 5 ug of ECHS1 antibody. Western blot analysis was performed using ECHS1 antibody.")
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![Immunohistochemistry Annexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Annexin_A1_AF3770_Immunohistochemistry_10404.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")