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CPT1B Antibody

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Western Blot: CPT1B Antibody [NBP1-59576] - Sample Type : 1: 45ug human capan1 cell lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti-rabbit HRP Secondary Antibody Dilution : 1:5000 Color
Immunohistochemistry: CPT1B Antibody [NBP1-59576] - searcher: Dr. Pankaj Kumar Singh, UNMC, Omaha, NE Application: IHC Species+tissue/cell type:Species+tissue/cell type: Human Capan1 cells Primary antibody dilution: ...read more
Western Blot: CPT1B Antibody [NBP1-59576] - Titration: 0.2-1 ug/ml, Positive Control: HT1080 cell lysate.
Western Blot: CPT1B Antibody [NBP1-59576] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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CPT1B Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to CPT1B(carnitine palmitoyltransferase 1B (muscle)) The peptide sequence was selected from the middle region of CPT1B. Peptide sequence DLEMQFQRILDDPSPPQPGEEKLAALTAGGRVEWAQARQAFFSSGKNKAA. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPT1B
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin
  • Western Blot 1.0 ug/ml
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-59576 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31013835).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for CPT1B Antibody

  • carnitine O-palmitoyltransferase 1, muscle isoform
  • carnitine O-palmitoyltransferase I, mitochondrial muscle isoform
  • Carnitine O-palmitoyltransferase I, muscle isoform
  • carnitine palmitoyltransferase 1B (muscle)
  • Carnitine palmitoyltransferase 1B
  • Carnitine palmitoyltransferase I-like protein
  • CPT I
  • CPT1B
  • CPT1M
  • CPT1-M
  • CPT1-MMCCPT1
  • CPTI
  • CPTI-M
  • EC 2.3.1
  • EC 2.3.1.21
  • FLJ55729
  • FLJ58750
  • KIAA1670
  • MCPT1
  • M-CPT1

Background

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CPT1B Antibody (NBP1-59576)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: WB, Western Blot.


Filter By Application
WB
(3)
Western Blot
(2)
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Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using NBP1-59576 Applications Species
de Souza SLB, Mota GAF, da Silva VL et al. Effects of early exercise on cardiac function and lipid metabolism pathway in heart failure J Cell Mol Med 2023-08-31 [PMID: 37654004] (Western Blot) Western Blot
Zhang F, Liu L, Xie Y et al. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-? pathway Open Med (Wars) 2022-02-22 [PMID: 35799598] (Western Blot) Western Blot
Pellegrin M, BouzourEne K, Aubert JF et al. Impact of aerobic exercise type on blood flow, muscle energy metabolism, and mitochondrial biogenesis in experimental lower extremity artery disease Sci Rep 2020-08-20 [PMID: 32820213] (WB, Mouse) WB Mouse
Chacinska, M;Zabielski, P;Ksiazek, M;Szataj, P;Jarzabek, K;Kojta, I;Chabowski, A;Btachnio-Zabielska, AU; The Impact of OMEGA-3 Fatty Acids Supplementation on Insulin Resistance and Content of Adipocytokines and Biologically Active Lipids in Adipose Tissue of High-Fat Diet Fed Rats Nutrients 2019-04-12 [PMID: 31013835] (WB, Rat) WB Rat
de Toledo Frias F, Rocha KCE, de Mendonca M et al. Fenofibrate Reverses Changes Induced By High-Fat Diet On Metabolism In Mice Muscle And Visceral Adipocytes J. Cell. Physiol. 2017-09-19 [PMID: 28926107] (Mouse) Mouse
Brittain EL, Talati M, Fessel JP et al. Fatty Acid Metabolic Defects and Right Ventricular Lipotoxicity in Human Pulmonary Arterial Hypertension. Circulation 2016-05-17 [PMID: 27006481] (WB, Human)

Details:
The Novus CPT1B antibody was used in western blotting of human tissue homogenates
WB Human

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CPT1B
Entrez
Uniprot