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GCDH Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Analysis in human liver and testis tissues using NBP2-48549 antibody. Corresponding GCDH RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human kidney, liver, pancreas and testis using Anti-GCDH antibody NBP2-48549 (A) shows similar protein distribution ...read more
Independent Antibodies: Western Blot: GCDH Antibody [NBP2-48549] - Analysis using Anti-GCDH antibody NBP2-48549 (A) shows similar pattern to independent antibody NBP2-48907 (B).
Immunocytochemistry/ Immunofluorescence: GCDH Antibody [NBP2-48549] - Staining of human cell line U-2 OS shows positivity in mitochondria.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human testis shows weak granular cytoplasmic positivity.
Immunohistochemistry-Paraffin: GCDH Antibody [NBP2-48549] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GCDH Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GCDH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GCDH Recombinant Protein Antigen (NBP2-48549PEP)

Reactivity Notes

Mouse (87%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GCDH Antibody

  • ACAD5
  • EC 1.3.99
  • EC 1.3.99.7
  • GCD
  • glutaryl-CoA dehydrogenase
  • glutaryl-CoA dehydrogenase, mitochondrial
  • glutaryl-Coenzyme A dehydrogenase

Background

GCDH is encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GCDH Antibody (NBP2-48549) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GCDH