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ECHS1 Antibody

Images

 
Independent Antibodies: Western Blot: ECHS1 Antibody [NBP1-87078] - Analysis using Anti-ECHS1 antibody NBP1-87078 (A) shows similar pattern to independent antibody NBP1-87079 (B).
Immunocytochemistry/ Immunofluorescence: ECHS1 Antibody [NBP1-87078] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human kidney.
Western Blot: ECHS1 Antibody [NBP1-87078] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human skeletal muscle shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining in human liver and skeletal muscle tissues using anti-ECHS1 antibody. Corresponding ECHS1 RNA-seq data are presented ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human cerebral cortex, kidney, liver and testis using Anti-ECHS1 antibody NBP1-87078 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human testis.
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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ECHS1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ECHS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
ECHS1 Protein (NBP1-87078PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ECHS1 Antibody

  • EC 4.2.1
  • enoyl CoA hydratase, short chain, 1, mitochondrial
  • enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
  • Enoyl-CoA hydratase 1
  • enoyl-CoA hydratase, mitochondrial
  • SCEHEC 4.2.1.17
  • Short-chain enoyl-CoA hydratase

Background

ECHS1 is encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ECHS1 Antibody (NBP1-87078) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ECHS1