Immunohistochemistry-Frozen: EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor® 488] [NB100-1869AF488] - EAAT-1/GLAST is expressed in inner phalangeal and inner border cells adjacent to inner hair cells in 1 week old ...read more
Flow Cytometry: EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor® 488] [NB100-1869AF488] - An intracellular stain was performed on Hek293 cells with EAAT1/GLAST-1/SLC1A3 Antibody NB100-1869AF488 (blue) and a matched ...read more
Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor 488] [NB100-1869AF488] - Intracellular staining of HEK293 cells (1 x 10^6 cells/ml) with EAAT-1 antibody (orange) stained at a dilution of 1:500. Shown ...read more
Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for EAAT1/GLAST-1/SLC1A3 Antibody [Alexa Fluor® 488]
solute carrier family 1 (glial high affinity glutamate transporter), member 3
Solute carrier family 1 member 3
Background
Excitatory amino acid transporter 1 (SLC1A3), also known as sodium-dependent glutamate/aspartate transporter 1, GLAST-1, solute carrier family 1 member 3, EAAT1, and EA6, transports and regulates L-glutamate, D-aspartate, and L-aspartate. SLC1A3 ends the glutamate postsynaptic actions by removing remaining glutamate from the cleft and by reducing extracellular glutamate. SLC1A3 is a member of a family of high affinity sodium-dependent transporter molecules that regulate neurotransmitter concentrations at the excitatory glutamatergic synapses of the mammalian central nervous system. In addition, SLC1A3 may also be important for the prevention of glutamate excitotoxicity. The SLC1A3 protein is located on the cell membrane. SLC1A3 is prominently expressed in the cerebellum, frontal cortex, hippocampus and basal ganglia and is also reported to be found in heart, placenta, lung and striated muscle. The SLC1A3 protein is subject to glycosylation and phosphorylation. SLC1A3 has two known isoforms (59.5 and 55 kDa) formed by alternative splicing. Mutations in the SLC1A3 gene have been linked to episodic ataxia and hemiplegia (PMID: 16116111) while other mutations result in a form of SLC1A3 (E219D) which influences Tourette syndrome (PMID: 21233784).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for EAAT1/GLAST-1/SLC1A3 Antibody (NB100-1869AF488). (Showing 1 - 2 of 2 FAQ).
Can I use this antibody for isolating live cells by using FACS sorting? GLAST is a membrane transporter. So I expected that we can sort live cells using this antibody. Can we?
Whether the antibody can be used with live cells, that would depend on two criteria: the presence of any preservative and endotoxin levels. The NB100-1869AF488 does not contain preservatives; thus, this criterion is good for you cell sorting experiments. In terms of the presence of endotoxin, the harmful effect(s) will depend on the cell types (the resistance/tolerance to the endotoxin). For example, blood cells and primary cells should be readily activated by endotoxin, but other cell lines may not be so reactive. Since we don't know what kind of cells that will be used, it will need wet-bench tests to find out.
Do you offer cat# NB100-91662 and NB100-1869AF488 labelled with Cy5 or APC ?
NB100-1869 is validated for Flow, both types of IHC (frozen and paraffin), and ICC/IF ... and already has several conjugates available.We could add this antibody conjugated to APC or Cy5 into our system if interested. NB100-91662 is not validated for Flow but has been validated for IHC (parrafin only IHC-P) ... and already has several conjugates available. We could add both conjugates, but if these are going to be used for Flow it would not be guaranteed.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.