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DPEP1 Antibody

Images

 
Western Blot: DPEP1 Antibody [NBP1-86087] - Analysis in control (vector only transfected HEK293T lysate) and DPEP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DPEP1 Antibody [NBP1-86087] - Staining human cell line Hep G2 shows positivity in nucleus and cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human testis.
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human kidney shows high expression.
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human lymph node shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining in human kidney and lymph node tissues using anti-DPEP1 antibody. Corresponding DPEP1 RNA-seq data are presented for ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human colon, kidney, liver and testis using Anti-DPEP1 antibody NBP1-86087 (A) shows similar protein distribution ...read more
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human colon.
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human liver.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DPEP1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: SLIGVEGGHSIDSSLGVLRALYQLGMRYLTLTHSCNTPWADNWLVDTGDSEPQSQGLSPFGQRVVKELNRLGVLIDLAHVSVATMKATLQLSRAPVIFSHSSAYSVCASRRN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DPEP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
DPEP1 Protein (NBP1-86087PEP)
Publications
Read Publication using NBP1-86087.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DPEP1 Antibody

  • dehydropeptidase-I
  • dipeptidase 1 (renal)
  • dipeptidase 1
  • DPEP1
  • EC 3.4.13.19
  • HRDP
  • MDPMBD1
  • Microsomal Dipeptidase
  • RDPMicrosomal dipeptidase
  • Renal Dipeptidase

Background

DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renalmetabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982 (PubMed 6122685)). DPEP1 isresponsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984(PubMed 6334084)). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probablefunction was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibitselective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990 (PubMed2303490)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DPEP1 Antibody (NBP1-86087)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DPEP1
Entrez