![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0005.jpg "Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, Simple Western, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies. Accession # P27718 |
| Specificity | Specific for endogenous levels of the ~55 kDa Dopa Decarboxylase/DDC protein. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDC |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody can be used in Western blot where a single band at 55 kDa is seen. Immunohistochemistry-Paraffin was reported in scientific literature. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
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| Publications using NB300-174 | Applications | Species |
|---|---|---|
| Fathi A, Bakshy K, Zieghami L et al. Diverging Parkinsons Disease Pathology between patient-derivedGBAN370S, LRRK2G2019Sand engineeredSNCAA53TiPSC-derived Dopaminergic Neurons bioRxiv 2023-01-07 (Western Blot, Human) | Western Blot | Human |
| Valsecchi V, Errico F, Bassareo V et al. SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy Communications biology 2023-11-13 [PMID: 37957344] (WB, Mouse) Details: 1:1000 WB dilution |
WB | Mouse |
| Blechingberg J, Holm IE, Johansen MG et al. Aromatic l-amino acid decarboxylase expression profiling and isoform detection in the developing porcine brain. Brain Res 2010-01-13 [PMID: 19857468] (IHC-P, WB, Porcine) | IHC-P, WB | Porcine |
Secondary Antibodies |
Isotype Controls |
Research Areas for Dopa Decarboxylase/DDC Antibody (NB300-174)Find related products by research area.
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![Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Western-Blot-NB300-174-img0004.jpg "Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.")
![Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/Dopa-Decarboxylase-DDC-Antibody-Simple-Western-NB300-174-img0003.jpg "Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.")
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